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Questions related from Zahra Damirchi
Hello everyone, I'm seeking a primary antibody that can specifically identify and detect Infliximab using the Western blot technique. The challenge I'm encountering is that the majority of...
14 July 2025 8,749 2 View
Hello. My polyacrylamide gel becomes cloudy after de staining with a solution consist of (50% miliQ water + 40% methanol + 10% acetic acid) and the background of my gel does not become completely...
03 May 2024 943 2 View
Hello, I need a detailed protocol for Sulfo-Phospho-Vanillin method to quantifying lipid residue in our product (that its host is a yeast: pichia pastoris). I chose this protocol instead of Folch...
02 February 2024 3,878 0 View
Hello. I mistakenly bought bPEI 60 MN, 50wt instead of bPEI 10 MN, And now I need 75 mg/ml of bPEI 10MN solution. 1) Does anyone know how can I prepare my desired solution from PEI 60 MN, 50 wt?...
22 October 2023 2,020 5 View
Hello. In my reference article, polyethylene imine (PEI-CAS No.: 9002-98-6) with a molecular weight of 100 kDa is used to functionalize mesoporous silica nanoparticles (MSN). My challenge right...
28 August 2023 9,478 3 View
Hello. I need to 100 ul from 75mg/ml polyethyleneimine solution (M.N. 60000 and 50 wt.% aqueous solution). Its a very viscose liquid and with CAS No [9002-98-6]. I have 3 challenges: 1-what is...
07 August 2023 8,679 1 View
I use the sol-gel method for synthesis and once I got a negative 40 potential, once a negative 80, and another time a negative 100. Also, I have never obtained a nanoparticle size of less than 200...
24 February 2023 1,559 2 View
Hello, I have a question about the reason for the difference between the B factor (temperature factor) in NMR and Crystallography, Why B Factor is usually zero in NMR?
30 November 2022 6,381 0 View
Hello, I use from Biorad Precision Plus Protein dual-color standard (MW marker), but the molecular weight that is estimated with this marker isn't usually precise. to confirm, I used from USP...
20 April 2020 3,303 5 View
Hello. I used from Thermo Scientific BCA kit and Sigma protein STD 2 mg/ml (0-2000 ug/ml) and prepared a lot of dilutions from my sample (up to 1-64). I had a different final concentration on my...
19 March 2020 685 3 View
1) Hi, Do you know that NIBS 98/574 standard is used in SDS-PAGE? (in this standard COA, it's not mentioned that the above standard is suitable for SDS-PAGE or not?) 2) why my recombinant human...
29 December 2019 2,075 5 View
Hello, I am looking for a suitable antibody for hGH. I wanna to confirm my result with a confirmatory test such as Western Blot. Thank you.
02 July 2019 6,389 3 View
