I am trying immunofluorescent staining of mouse brain tissues for CD4 and CD8. Does anyone here know any antibody that worked for them in fresh frozen mouse brain tissue?
Few information:
1. Trying to stain for CD4 and CD8.
2. Tissue has tumor so I expect CD4 and CD8 should be there.
3. I am using fresh/snap frozen tissue (it was not fixed). Fixing with Methanol during staining.
4. Blocking using 5% BSA and 0.1% Triton X100.
5. Tried couple antibodies with different dilutions. No luck yet.
Name of the antibodies I have tried:
1. CD8, 53-6.7, ebioscience.
2. CD8, 53-6.7, Novus
3. Cd8a, D4W2Z, Cell signaling technology
4. CD4, EPR19514, Abcam
5. CD4, pAb, Novus
Dilutions tried are 1/100 and 1/500 in 5% BSA.
I will really appreciate if anyone share the name of antibody and/or protocol that they have used to stain the mouse brain tissues for CD4 and/or CD8.
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