04 October 2013 2 9K Report

I want to stain neurons in the rat hippocampus for aromatase, but the antibody, which I used, have a strong background and I have observed no positive staining in neurons (paraffin sections).

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How looked like the Pagan-Christian relationships, how their wedding rituals has changed during the 4th century and visual language was used for this?

How the relation between Pagan parents and Early Christian children generation looked like? How the wedding ritual during the 4th century has changed? And how was the visual language and...

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Which p-value is the right one in a subgroup meta-analysis?

Could someone explain to me why the p-value in the right column of the forest plot is different than the p-value in the test for effect in the subgroup? I thought that these two p.values should...

11 August 2023 1,776 6 View

What causes the interference fringes in the Raman spectrum?

Hello, I was measuring my sample - cellulose impregnated with polyethyleneimine, on a Raman microscope that has back-illuminated CCD. I used 633 nm excitation laser. In my spectrum, I got wavy...

29 January 2022 2,416 3 View

Has anybody had any experiences concerning switching from TRIZOL RNA extraction to using a commercially available kit?

I would like to extract RNA from tissue, but the samples are currently stored in TRIzol. How should I treat the samples if I want to use them with a kit? Can TRIzol somehow interact with the...

02 September 2021 6,838 3 View

What causes SERS intensity to be low at high solution concentration?

I prepared polydopamine layers with silver nanoparticles and tested for SERS. SESR intensity of the lowest rhodamine B solution concentration (10-7 mol/l ) is higher than the one of the most...

13 June 2020 7,952 3 View

How to increase cell yield after dissociation of breast tumors using Tumor Dissociation Kit?

Hi, we are preparing single cell suspensions from breast tumors for mass/flow cytometry analysis. We have tried different dissociation protocols (collagenase I, dispase II, etc) but none of...

18 September 2019 9,194 2 View

Cell line which sprouts neurites?

Hello, we are about to start an experiment on Perineural Invasion of cancer cells. I am wondering, if you have any good experience with any cell line which sprouts neurites. I have to admit I am...

25 February 2019 8,832 4 View

Bacterial contamination when inducting S. cerevisiae strain EBY100 for scFv display?

Hi all, Did somebody experience bacterial contamination problems when inducing scFv display on the EBY100 with galactose containing media (SG-CAA)? Before induction, yeast is thawed and...

20 September 2018 4,486 4 View

Does anybody experience with RNAlater™-ICE Frozen Tissue Transition Solution and plant tissue?

Does anybody have an experience with stabilizing plant leaf tissues with RNAlater™-ICE Frozen Tissue Transition Solution? Is it necessary to homogenize the plant tissue for RNA isolation before...

27 March 2018 2,028 3 View

How to isolate bacterial RNA without lysis by lysozym?

I have frozen (-80°C) samples of bacteria (both gram-positive and negative) in PBS. I would like to isolate RNA from those samples, but without use of lysozym (part of pre-lysis treatment in Roche...

02 January 2017 1,827 2 View

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