I would like to extract RNA from tissue, but the samples are currently stored in TRIzol. How should I treat the samples if I want to use them with a kit? Can TRIzol somehow interact with the buffers in the kit and I should wash it out?
Thank you for any suggestions!
I never had to remove Trizol from my samples, but I had many issues with RNA later. What worked best for me was to wash the tissues two times in a cold RNAse-free PBS solution. As a result, I obtained great qualities and RIN. Another option that could work for you is simply using Trizol instead of the kit's lysis buffer. I usually homogenize the samples with Trizol, add chloroform and remove the supernatant after centrifugation. After that, I follow the manufacturer's instructions.
I hope this was helpful to you!
It may depend on what you intend as downstream applications of your extracted RNA, but perhaps what you could try is TRIzol extraction followed by RNA purification. Taking a look at the TRIzol plus RNA purification kit available from invitrogen/Thermo Fisher might be helpful: https://www.thermofisher.com/order/catalog/product/12183555#/12183555
Gabriela Dalmaso de Melo Hello, thank you for the answer. I have a bad experience with traditional TRIzol - chloroform isolation. I am working with quite small samples, therefore the removal of the RNA is difficult. I do not have a nice DNA pellet even though I added glycogen. That is why I want to switch methods and try kit instead.
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