Currently, different in vitro models of COPD are applied in primary studies. The main question is "what is the best and most simple and also most reliable way to mimic the COPD situation in human airway epithelium cell line?"especially, when we want to investigate the inflammatory mediators. What is the best cell line to work on (individual or co-culture system)?
Dear Mahyar Agapour - there is a recent paper from Belfast on this subject using cigarette smoke extract - which seems reasonable given that cigarette smoke is the causative agent for the majority of COPD ( paper attached). Please remember that there is increasing evidence that COPD is a SYSTEMIC disease so you may find it useful to use other cell lines too to examine these other effects. Hope this helps. Best wishes
I don't think you can call it COPD if it's in vitro. Sure you can expose cells to cigarette smoke extract, but that's really just a model of smoke exposure.
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Cell Biology
- Culture Cells
- Cell Line