The microfluidic chip is consisted of several grooves, the sizes are almost 50*50 um. Starting the test i have trouble removing the air bubbles which are trapped inside these grooves. I would really appreciate it if any one could teach me a practical way to move forward. thank you.
Pressurizing both inlet and outlet of the chip will help remove the air bubbles. This technique is more efficient in porous chips like PDMS chips since the air will go through the material.
Another way is using of a bubble trap in line before the chip, removing all the bubbles which could be inserted in the fluidic path.
thank you Prajal Chettri i really appreciate your time.
the air bubbles are not from the inlet so the bubble trap in line before chip would not help.
after fabrication process the microgrooves inside the microfluidic chip, are filled with air and through the experiment it seems that the fluid can not enter the grooves. i have used ''Pressurizing both inlet and outlet of the chip'' and it did not help either, since the groove walls are really thin i guess.
again thank you for your suggestions.
You can try using ethanol or isopropanol to flush the device first. This should help to get rid of the bubbles. Then, you can flush your buffer to remove ethanol/isopropanol before loading the sample.
Iman Ramazani Sarbandi Could you solve your problem with ethanol or IPA perfusion before perfusion with medium or buffer?
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