14 Questions 16 Answers 0 Followers
Questions related from Viraj Mehta
Dear all, I am attaching pictures of microfluidic culture of MCF7 on day 2, day 3 and day 5. I found on day 2 the spheroids had tight boundary but the next day all of a sudden the spheroids are...
20 December 2021 6,520 0 View
I have recently started cell seeding inside my PDMS microfluidic device with syringe pump. I am actually trying to form tumor spheroids inside microwells coated with pluronics. However, the MCF7...
29 October 2021 6,526 5 View
Dear all I am looking for autoclavable tubings for dynamic cell perfusion studies. Can anyone suggest any such product available in India? Thank you in advance!
22 June 2021 8,485 0 View
Hi all, I am trying to culture tumor spheroids inside my PDMS based microfluidic devices. However, as soon as I complete 24 hrs of dynamic perfusion, while removing the tubes I found the...
21 February 2021 9,782 4 View
I found my PDMS microfluidic devices turned turbid after 100% IPA perfusion to remove the bubbles. Once I perfused DI water to replace IPA, next day I found the PDMS was hazy. Is there any...
11 January 2021 2,848 3 View
Dear sir/ma'am, I have recently tried coating of Plasma bonded PDMS microfluidic devices with pluronics F-108. After introducing F 108 in microchannels, I incubated my devices at 37 degrees...
11 January 2021 2,559 0 View
I have 3 drugs mixed in a solution coming out from a microfluidic device. I know the concentration of each drugs at inlet. If I have absorbance data of each inlet drug solutions, how to estimate...
14 October 2020 1,103 3 View
Hi, Hope you are all doing well. My microfluidic device is having pipette tips projecting up for gravity based flow perfusion. Because of which, I can not accommodate the chip inside standard 100...
06 April 2020 9,207 1 View
I am using UV/ozone (ambient air) to treat my pdms to bond them. We have been using it since last 9 years. Recently, I kept two pdms devices inside for treatment, one got treated with contact...
23 January 2020 2,888 0 View
I have microfluidic device which has mold made up of acrylic which has been micro milled. Due to micro milling, the surface is rough and when I cast PDMS over it, the PDMS surface also becomes...
20 September 2019 4,135 4 View
Hi, I wanted to know concentration of (dissolved) oxygen in (mg/L) in cell culture experiments in standard incubator condition. Your help will be very much appreciated. Thank you.
05 August 2019 8,354 2 View
Hi all, I am cleaning my microfluidic devices using these steps: 1. manually perfusing ethanol 2. manually perfusing PBS 3. Keep in desiccator for drying 4. UV under culture hood for 30...
26 July 2019 3,194 3 View
Dear all, I have two molecules having the same solvent (say water) but having Diffusion coefficient D1 and D2 in water. So If I am having microfluidic mixer, do I need to consider the same...
17 July 2019 2,482 3 View
I want to control pressure of 'Biobots' bioprinter with respect to time according to say ramp input profile or sinusoidal profile. Every time I set a constant pressure value before staring 3D...
09 November 2018 5,796 1 View
