What is the behavior of the chitosan when the pH changes?

How to assess the osmotic power of a substance?

Hello, I suspect that a molecule I am using is causing bacteria to experience osmotic shock. What chemical properties should I look for to compare this capability with those of other substances?...

06 August 2024 977 1 View

How to check pH of a high strength hydrogel?

We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...

30 July 2024 942 2 View

EDTA buffer solution?

Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.

29 July 2024 4,374 1 View

Whether i can preserve leaves samples in 2.5% glutaraldehyde in 0.05 M phosphate buffer, pH 7 for coming several weeks for further LM study ?

I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.

29 July 2024 8,560 0 View

Does anybody know why my chitosan/pva/gelatin hydrogel dissolve in PBS?

My final research is about making chitosan/pva hydrogel for wound healing using gelatin as crosslinker. Last month, i made the thin film hydrogel and it turn well. It was okay. Last week, i make...

28 July 2024 2,794 6 View

How can I regenerate a SCX column modified with Sulfonate group (Thermo 054995)? The colmun has lost its Resolution and peak to valley ratio?

The column is used in Ion Exchange Chromatography of Monoclonal antibodies and the method of analysis is based on pH gradint.

13 July 2024 7,462 1 View

How i figuer out the weight of curcumin powder?

I am trying to load curcumin emulsion in 4% chitosan gel but i don not know how many mg powder is needed ?

10 July 2024 2,462 1 View

My Cyclic voltammetry curve doesn't show cathodic peak of Cu2+ to Cu+. how to solve this problem?

I performed CV of Cu-MOF in a buffer solution of pH 8, and we did not find any peak corresponding to Cu2+ to Cu+. What is the reason behind this? Please give your valuable suggestions.

08 July 2024 2,388 4 View

Is it possible to generate a (semi-)permanent "transcription bubble" within a replicable plasmid?

I was wondering if it is possible to form a permanent open "ssDNA bubble" similar to a transcription bubble (>13 nucleotides) within E. coli. These criteria are important: 1. Open ssDNA...

07 July 2024 8,275 5 View

What are the strategies to improve the stability of chitosan nanoparticles in DMEM (cell culture media)?

Hi, I am synthesising chitosan nanoparticles using the ionic gelation method and I am characterising the nanoparticles in distilled water and in DMEM (cell culture), however the zeta potential in...

01 July 2024 1,526 1 View