Recently I have read a lot of papers referring to the subcellular distribution and chemical forms of Cd (or other heavy metals) in different plant organs to identify the sepcific phytotoxicity of Cd. Most of them used the different centrifugation method, by which the plant samples are homogenized with a mixture of solutions and centrifugated in different rpm and time. However, I am still confused about the principle of methods. How can the three subcellular fractions (cell wall, organalle, and soluble fraction) be seperated by centrifugation with different rpm and time? On what basis can this happen? Hope somebody can answer me. Thank you.
It's likely that the Cd ions are getting bound to some protein lipoprotein membranes that cause variation in their molecular masses. This results in sedimentation of Cd-membrane complexes at diff rcf or rpm value during centrifugation.
Shamsher S. Kanwar Thank you very much. Can you provide some related paper or other evidence which can support your assumption?
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