What is the basic difference between wheat derived phytase and microbial phytase?

Ajeet Singh @Ajeet-Singh-13

02 August 2019 0 9K Report

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Why is electronic energy 0.000 for liquid crystal compounds and is invariable with temperature in Guassian 09 software?

Are there any suggestions or insights you can provide to help address this problem

06 August 2024 1,443 2 View

Please explain how the plastic input value should be considered from the true stress-strain curve for the bilinear elastoplastic material model ?

I am working on Abaqus/Explicit(Quasistatic ) for the deformation of the auxetic structure model. Please explain how the plastic input value should be considered from the true stress-strain curve...

05 August 2024 454 3 View

How to increase citation in Research Gate?

How to enhanced h-index in Research Gate?

04 August 2024 3,368 4 View

How to do wavelet transform of EXAFS data?

I want to do wavelet transform of some compounds for their comparative study? Kindly help me in this regard and provide with proper procedure.

31 July 2024 4,170 0 View

Following click reaction in cell lysates, protein is immobile and remains at the top of the gel in SDS-PAGE?

I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...

29 July 2024 950 4 View

How to use energy flexibility in inventory modeling?

29 July 2024 2,192 5 View

I want to get updates regarding international law, international trade law, international institutions and human rights etc?

Please send me updates regarding the above-mentioned areas in the question mark?

28 July 2024 5,197 3 View

What is the principle/mechanism behind aging of carbon (graphite) containing refractory mix for isostatically pressed refractories?

The resin bonded carbon containing refractories are aged before use. How time of aging is determined? And mechanism behind aging.

23 July 2024 3,205 0 View

How to remove cell debris after tissue dissociation?

I'm facing issues with the tumor dissociation protocol for GBM. I use collagenase/dispase with DNase to dissociate my tumor sample. However, even after incubating it for 60mins, I do not get a...

22 July 2024 412 3 View

What methods can be used to minimize environmental variability in breeding trial ?

In breeding trials, environmental variability can significantly affect the results, making it challenging to accurately evaluate the performance of different lines and hybrids.

22 July 2024 3,961 4 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Does anyone notice the tumor regression impact of Hepa1-6 cells in HCC orthotopic mice model?

To establish an orthotopic HCC model, C57BL/6 mice (male, 6-8 weeks) were orthotopically implanted with 3x106 Hepa1-6 cells (Over-expressed with a human gene) in 20uL Matrigel into the left lobe...

18 July 2024 9,177 2 View

What do I do with dried urine samples?

I placed mice in metabolic cages to collect urine samples for analysis. However, the majority of the samples have dried up. Is there any way to retrieve the urine in those tubes without...

14 July 2024 6,724 0 View

How long can I store mouse tissues in neutral buffered formalin for, and how often should I add fresh formalin?

My study uses C57BL/6J mice and I am required to store portions of the important organs in neutral buffered formalin (NBF). Could I potentially keep those tissues in the formalin for a few weeks...

06 July 2024 8,360 2 View

Apart from basic bone remodeling markers, which other blood biomarkers do you think have potential to predict fractures in older adults?

Currently, there are numerous blood biomarkers that have been linked to fractures and increased fracture risk (for example, IL-6). Considering the current evidence, which ones do you think are the...

10 May 2024 6,097 2 View

Why are the mice in my control group mildly hyperglycaemic?

I am currently working towards developing a model for type 2 diabetes, using fructose and streptozotocin (STZ). Prior to injecting my C57BL/6 mice with STZ, we tested blood glucose with a...

08 May 2024 3,947 2 View

What is the good dilution factor for mouse serum in ELISA?

I am doing IL6 ELISA to measure mouse serum IL6, but my ODs are so low even for mouse on two weeks high fat diet. My standard curve is good enough as a positive control.

28 April 2024 8,922 2 View

Can I use syngeneic mice model to study the role of Cancer Associated Fibroblast (CAF) for ovarian cancer ?

Can I use syngeneic mice model to study the role of CAF ? I have gonr through recent papers related to this and found most of the papers are worked with Balb/c nude mice with xenograft. Is it...

21 April 2024 7,980 0 View

What are alternative methods to ELISA analysis of serum and tissue homogenates for the quantification biomarkers?

I am working with C57BL/6 mice for the development of a T2DM mouse model. During the course of this experiment, the mice will have their urine, blood, and tissue samples collected and examined for...

16 April 2024 530 2 View

Optimal concentration of IL-6 for stimulation?

Hi! I'm trying to stimulate cells with IL-6 (in order to perform a qPCR) but i can't find a proper protocol. Any suggestions?

03 April 2024 379 3 View