I have subcutaneously injected 5 million Kasumi-1 cells into NOD/SCID mice. I wish to know how long does it take to form tumors.
Check Fig. 6A in the following paper.
Article Antileukemic Activity of 2-Deoxy-D-Glucose through Inhibitio...
Hi Shraddha, I once utilized lymphoma cell lines to form sub-Q tumors in NOD/SCID mice. It will take about 4-6 weeks to form a tumor if you inject 3 million cells per mouse.
Hi! I wish to perform FACS of cells isolated from a subcutaneous tumor. Kindly suggest methods that would be suitable for making single-cell suspension of the tumor excised from mice
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Hi! I wish to check the tumorigenicity of leukemic stem cells sorted from a specific AML cell line for which I need to decide three dilutions of cells to be injected. Though its a variable answer,...
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I wish to maintain my sorted leukemic stem cells as "stem cells" and keep them from differentiating. Kindly suggest some ways in which this can be done. (Except for media without serum).
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Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?
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Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
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We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
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Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...
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I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
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Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...
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Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
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I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...
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