What is the average time duration taken by Kasumi-1 (AML cell line) cells to form tumors after subcutaneous injection into NOD/SCID mice?

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Can someone suggest a method for enzymatic dissociation of a subcutaneous tumor followed by preparing a single-cell suspension of it?

Hi! I wish to perform FACS of cells isolated from a subcutaneous tumor. Kindly suggest methods that would be suitable for making single-cell suspension of the tumor excised from mice

03 April 2018 6,558 3 View

What is the minimum no. of leukemic stem cells that can form tumor when injected into NOD/SCID mice subcutaneously?

Hi! I wish to check the tumorigenicity of leukemic stem cells sorted from a specific AML cell line for which I need to decide three dilutions of cells to be injected. Though its a variable answer,...

08 September 2017 3,029 2 View

Which media/ conditions can be used to prevent cancer stem cells from differentiating?

I wish to maintain my sorted leukemic stem cells as "stem cells" and keep them from differentiating. Kindly suggest some ways in which this can be done. (Except for media without serum).

08 September 2017 9,841 3 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Julie Joseph

Check Fig. 6A in the following paper.

Article Antileukemic Activity of 2-Deoxy-D-Glucose through Inhibitio...

Han Zhang

Hi Shraddha, I once utilized lymphoma cell lines to form sub-Q tumors in NOD/SCID mice. It will take about 4-6 weeks to form a tumor if you inject 3 million cells per mouse.