Shraddha Thakkar

4 Questions 2 Answers 0 Followers

Questions related from Shraddha Thakkar

Can someone suggest a method for enzymatic dissociation of a subcutaneous tumor followed by preparing a single-cell suspension of it?

Hi! I wish to perform FACS of cells isolated from a subcutaneous tumor. Kindly suggest methods that would be suitable for making single-cell suspension of the tumor excised from mice

04 April 2018 6,547 3 View

What is the average time duration taken by Kasumi-1 (AML cell line) cells to form tumors after subcutaneous injection into NOD/SCID mice?

I have subcutaneously injected 5 million Kasumi-1 cells into NOD/SCID mice. I wish to know how long does it take to form tumors.

03 March 2018 8,128 2 View

What is the minimum no. of leukemic stem cells that can form tumor when injected into NOD/SCID mice subcutaneously?

Hi! I wish to check the tumorigenicity of leukemic stem cells sorted from a specific AML cell line for which I need to decide three dilutions of cells to be injected. Though its a variable answer,...

09 September 2017 3,015 2 View

Which media/ conditions can be used to prevent cancer stem cells from differentiating?

I wish to maintain my sorted leukemic stem cells as "stem cells" and keep them from differentiating. Kindly suggest some ways in which this can be done. (Except for media without serum).

09 September 2017 9,817 3 View