I got the EasySep monocyte isolation kit negative selection. After PBMC isolation the number of PBMC cells was about 3x10^7 cell/ml. What is the average number of monocytes that I am supposed to get after isolation by the kit?
I am a Product Manager at STEMCELL Technologies. I see our Scientific Support team was able to assist you with your inquiries.
For others who are curious about the answer to your question, recovery is affected by multiple factors including donor health, use of fresh vs frozen samples, and monocyte starting frequency. Please see our Tech Tip, Assessment of Your Starting Sample and Calculation of Recovery from Cell Isolation Procedures, for more information on how to calculate this for your sample.
If you'd like more help or want to discuss further, please reach out to our Product and Scientific Support team at [email protected].