What is the average bacterial load of M. tuberculosis in the saliva of an actively-infected patient?

08 August 2016 1 3K Report

I'm trying to validate a real-time PCR assay for TB in non-human primates. I need a reference concentration to prepare positive samples (using spiked negative samples).

Seema Simon

That bacteria is an airborne!!!!

Badges
Science topic

More Jose Sevilla's questions See All

Does anyone have qPCR Primers/Probes for Radfordia (fur mite) genus?

My laboratory is trying to develop a qPCR assay to detect rodent fur mites Radfordia affinis and Radfordia ensifera. However, there are no published nucleotide sequences in NCBI for either...

09 October 2017 8,972 7 View

TaqMan qPCR Assay Using a StepOnePlus - Has anyone experienced Ct values increasing at random for established controls?

Greetings. I'm running a TaqMan assay on the StepOnePlus R-T PCR System, using the TaqMan Fast Advanced Master Mix. I validated an assay that gives me very precise Ct values for positive controls...

09 October 2017 6,684 2 View

Are chicken polyclonal IgY ELISA coating antibodies compatible with Immulon 1B plates?

I tried using a GenwayBio ELISA kit (40-288-10066F), but I had no signal at 450 nm for samples, standards, or controls. Because of this, I suspect it may have been the coating process. I used the...

03 April 2017 9,272 3 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Are there any instruments for studying time similar to the way it is in space?

There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...

06 August 2024 7,102 0 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Why does the MFDFA algorithm need to calculate the profile of the time series?

As described in the Multifractal detrended fluctuation analysis (MFDFA) algorithm, it at first calculates the profile of the time series, and then other steps are operated on the profile....

05 August 2024 9,366 2 View

How can i do multivariate Time Series forecast using MLP, ANFIS and LSTM?

I need the python code to forecast what crop production will be in the next decade considering climate and crop production variables as seen in the attached.csv file.

05 August 2024 2,977 3 View

Is there any machine to do real time pcr?

I want to know how do you make real time pcr solation ? is there any machine to make it? thanks for answering

05 August 2024 1,660 0 View