Jose Sevilla

4 Questions 7 Answers 0 Followers

Questions related from Jose Sevilla

Does anyone have qPCR Primers/Probes for Radfordia (fur mite) genus?

My laboratory is trying to develop a qPCR assay to detect rodent fur mites Radfordia affinis and Radfordia ensifera. However, there are no published nucleotide sequences in NCBI for either...

10 October 2017 8,957 7 View

TaqMan qPCR Assay Using a StepOnePlus - Has anyone experienced Ct values increasing at random for established controls?

Greetings. I'm running a TaqMan assay on the StepOnePlus R-T PCR System, using the TaqMan Fast Advanced Master Mix. I validated an assay that gives me very precise Ct values for positive controls...

10 October 2017 6,673 2 View

Are chicken polyclonal IgY ELISA coating antibodies compatible with Immulon 1B plates?

I tried using a GenwayBio ELISA kit (40-288-10066F), but I had no signal at 450 nm for samples, standards, or controls. Because of this, I suspect it may have been the coating process. I used the...

04 April 2017 9,258 3 View

What is the average bacterial load of M. tuberculosis in the saliva of an actively-infected patient?

I'm trying to validate a real-time PCR assay for TB in non-human primates. I need a reference concentration to prepare positive samples (using spiked negative samples).

08 August 2016 3,471 1 View