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I am working with new fish cell lines especially from cold-water fishes, so how can we choose the best program and process to get cells with higher viability and transfection efficiency?...

16 September 2020 9,574 2 View

Which gRNA designing tool for Crispr experiment can be effective for the gene knock out experiments in fish cell lines specially like Atlantic Salmon?

Any suggestions for critical things to be considered for the fish cell line as it is for cold water fish which is completely different than other mammalian cell lines.

14 August 2020 7,083 2 View

CAN anyone explain me about cell staining, egg staining by using different dye such as methylene blue?

what is the principle behind staining what is its applications? why eggshell does not stain? why RBS does not stain? please explain to me everything. thank you

23 February 2020 7,247 2 View

Could anyone free for english editing service ??

One of my research article is rejected due to english language from Parasites and Vectors journal?? Is there anyone who able to assist me??

29 December 2019 5,207 8 View

Are there any computer science journals accepting reviewers?

Hi, I am looking for reviewing journals/articles in the following areas. 1) Computer Applications, Embedded Systems, Software Engineering, Communication Protocols, Operating Systems. 2)...

16 April 2018 5,894 1 View

Can anyone help me write a code for coverage and connectivity in wireless sensor network?

Can anyone help me write a code for coverage and connectivity in wireless sensor network?

18 July 2017 5,121 2 View

Can anyone send me Algorithm for crossover and mutation i.e. pseudo-code of crossover and mutation.

Can anyone send me Algorithm for crossover and mutation i.e. pseudo-code of crossover and mutation.

21 May 2017 5,091 1 View

How to cover and maintain connectivity in wireless sensor network using less number of sensors?

How to cover and maintain connectivity in wireless sensor network using less number of sensors?

28 August 2016 9,593 3 View

How to compute daily rainfall from hourly records?

07 July 2016 895 13 View

How are rRNA ,sRNA, and mRNA inter related?

mRNA is inhibited by sRNA and rRNA helps mRNA in translation. So what is the main link between these three types of RNA?

14 November 2013 3,866 2 View

Why can't make aluminum nitride film successfully by use sputter?

We use reactive sputtering process to make aluminum nitride film, and want to make c-axis aluminum nitride film on a silicon substrate, but the crystal orientation cannot be seen with...

03 March 2021 7,975 3 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

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02 March 2021 3,060 3 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

What thickness need for ITO to use as conductive layer?

I want to do RF sputtering of ITO on glass substrate using magnetron sputtering. what thickness do i need to make it good conductive layer to use it for PSC. Moreover, whats about thickness of...

01 March 2021 395 3 View

Adherance of Polyaniline coating over stainless steel?

Is the electropolymerisation of polyaniline coating over stainless steel (SS) via Cyclic voltammetry technique is really adherant? Else how to improve the adhesion of the coating, whether any...

01 March 2021 8,220 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View