Hi,
We are using Akta Avant 25 for R&D purposes. I am trying to optimize preparation of 1xPBS+0.09% Sodium Azide buffer by using the Quaternary valves to mix and prepare the buffer instead of making it manually each time. Current hand made buffers show a lot to lot variation in conductivity from 16.98mS/cm to 21.11mS/cm. This fluctuation is probably based on a few mls of 20xPBS added more or less based on different lots. From QC perspective, is this fluctuation significant? If I use the Quaternary valves on board the Akta FPLC to make the buffer and it shows this fluctuation, would it be of concern?
Thanks in advance for your reply.
I'd be happy with the quaternary valve made PBS if it showed a similar variation in conductivity, as 1x PBS is expected to be around 16.8 mS/cm. Surely the verification/validation docs for these units have such a listed spec. for the buffer mixing as its a selling point for these systems? Plus 25% on the high end could be an issue.
I can't see such a variation having much effect but this, like most things, is dependent on how tight your data needs to be. Checking for your "molecule of interest" (MOI) in prep fractions might see no impact from this variation but for a real analytic chemist/ QC analyst it might be too loose. What's the final application this spec. needs to support?
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