I am currently working on chitosan nanoparticles synthesis by ionic gelation method. I dissolve chitosan in 1% acetic acid (to make a solution of 1mg/ml) Regarding that I have some queries:
What is the ideal pH for chitosan nanoparticle synthesis?
Can anyone please share a protocol for chitosan nanoparticles synthesis?
How to increase the yield (final amount in milligram) of synthesized chitosan nanoparticles?
Is it necessary to maintain any specific pH (acidic/basic) of the STPP (cross linker) ?
The optimum pH ranges from 4.6 to 5; in cases of perparation of chitosan nanoparticles its highly recommended to use high purity chitosan, adding TPP at a proper ratio (1:4) and it should be added drop wise to chitosan solution under stirring