The attached provides some informations

Hi Nimish, I suggest you to check this discussion. https://www.researchgate.net/post/How_can_I_get_larger_quantity_of_chitosan_nanoparticles_using_ionotropic_gelation_method2

thank you Hugh J Byrne and Nirmla devi

pH should be in the range of 6-7, which can be done by using NaOH solution. For higher yield and good size of chitosan nanoparticles sodium-TPP solution should be added very slowly and drop-wise and temperature should be in the range of 25 degree Celsius.

Thank you Vikas Sharma

I have another query:

While synthesizing chitosan nanoparticles by ionic gelation method, is it necessary to maintain any specific pH (acidic/basic) of Sodium tripolyphosphate (STPP)? Is it ok to perform the chitosan nanoparticle synthesis at Room temperature in a fume hood OR 25 degree celcius temperature is necessary ?

NO, only Sodium TPP solution in distilled water is required (approx 2mg/ml). Chitosan nanoparticle at lower temperature will form small and uniform particles.

it is okay to prepare it at room temperature except in hot summer when temperature cross 40 degree.

Thank you Vikas Sharma

I thing 1% acetic acid is much higher, Chitosan can be dissolved easily in 0.2 to 0.5 % of acetic acid and is enough sufficient for ionic gelatin.

Thanks Vikas Sharma