Could you please tell, what percentage of cells are usually successfully infected(lytic infection) after first round of infection in bulk culture(after 50 minutes). When you work with DH5alpha E.coli and adding maltose in day culture.
Thank you.
When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...
03 March 2021 4,892 5 View
Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...
03 March 2021 6,193 1 View
We have synthesized various metal complexes with different environment around the metals. Now to see the application of synthesized complexes we need this study also. we are also interested in...
01 March 2021 1,899 2 View
Hello everyone. I have conducted the research on post harvest longevity of tomato crop using plastic packaging and different doses of chemicals as two different factors. The design of experiment I...
28 February 2021 8,189 3 View
The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....
27 February 2021 4,450 2 View
24 February 2021 5,378 1 View
In the PVD of TiN thin films (~100A), I've found the resistivity of the TiN films dropped from ~100 to 60um*cm with N2 flow increasing up to the metallic mode ending point; then the resistivity...
21 February 2021 1,864 2 View
Dear colleagues We are exploring possible data collection methods for environment and behavior research in the context of architectural environments. Have you conducted any Post Occupancy...
16 February 2021 2,575 4 View
To maintain the proper crop plan most of the times there is not enough time to make the field ready for the next crop. In this particular context , can bio-decompser be a solution? What about...
16 February 2021 7,068 24 View
Hi! Thank you for your time, attention, and support. I am currently using FEM solver Abaqus to perform a set of balloon inflation simulations, trying to understand the deformation shape, stress,...
14 February 2021 2,524 4 View
I have a model with bulk material (matrix) and inclusions and cohesive interface elements between both. For the bulk material, I have implemented a UMAT and for the cohesive interface elements, I...
20 February 2021 1,286 2 View
Hello, I'm trying to measure the physical and hydrological properties of organic substrate media. Currently, I'm referring The book Soil sampling and Methods of Analysis by Carter and Gregorich....
09 February 2021 3,233 8 View
My lab is using a lenti-X concentrator. And we are looking for a homemade recipe. I have tried this recipe....
08 February 2021 5,965 1 View
Hi, I want the use lithium niobate for the frequency conversion from 1030nm to 515nm. We want to order bulk material and machine it to the required dimensions. There are numerous suppliers of...
07 February 2021 6,786 2 View
My solid polymer electrolyte shows low bulk resistance show quite a low bulk resistance. However, the potential window (obtained from LVS) is very small. Please how can I improve the operating...
31 January 2021 3,340 9 View
Can we measure the bulk composition of biomolecules present within an algal sample directly by placing its powdered form on a FT-IR spectroscopy stage? Or does it require a particular kind of...
30 January 2021 2,303 4 View
Good afternoon, please how can you adjust pH of phage SM buffer? I presume that main ingredient that has influence of pH is Tris/HCl buffer, but according to literature its range of pH is from 7...
27 January 2021 5,767 3 View
Hi, I'm transducing Ly1 and L363 cell lines using our standard protocol for retroviral transduction. The cells are successfully transduced as evidenced by GFP expression. However, after 4-5 days...
26 January 2021 4,071 3 View
Hello everyone, I have to isolate DNA from Brucella phage , but before that, I have to concentrate the phage using PEG 8000. I have got protocol for concentrating the phage using PEG and TM buffer...
24 January 2021 2,767 6 View
I must put 50 kb plasmid into ecoli strain. I think 50 kb is too big for heat shock with ecoli. Is that correct? Will electroporation work? Or do I need to use cos sites and lambda phage...
22 January 2021 8,612 3 View