Sergei Smirnov

2 Questions 2 Answers 0 Followers

Questions related from Sergei Smirnov

Additional band near upper marker during NGS library preparation from FFPE?

Good day, could anybody suggest what could be the reason of a additional big band need upper marker? If we exclude contamination. I am using Agilent sure select XT protocol for targeted DNA seq of...

05 April 2022 6,966 0 View

What strain to use in cloning with araBAD promoter and gene of interest(1000bp) that is complementary to the region on the same plasmid?

I am trying to insert into PBR322 araBAD promoter with my gene of interest(it should be inducible). Could you please tell wether I could use DH5alpha strain as I was told it lacks arabinose...

29 January 2019 3,443 4 View