What is most useful IHC marker for uterus dendritic cells?

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I need help with Identification (misidentification) of European Pisidiidae & Sphaeriidae?

Hi everyone! I published an article summarizing my first experience in determining cryptic species of small bivalves, of course with errors. I will be very grateful and will thank you in future...

02 August 2024 6,582 11 View

Why typical glow discharge of TEM grids bring negative charge to the surface?

Hello everyone! Glow discharge treatment of TEM carbon-coated grids is a commonly used routine. Usually the grids are placed onto the cathode, and they are separated from the cathode by a glass...

08 April 2024 5,164 3 View

Why do identical sequences form a "comb-like" structure with non-zero branch lengths on the resulting phylogram in Mr.Bayes?

Hello, can anyone explain me why MrBayes gives long terminal branches for different speciomens with the same sequences? I am studying multigene phylogeny and for some individual nuclear gene tress...

28 February 2024 2,709 0 View

Does somebody know an open-source software for the video analysis of behaviour including manual scoring?

Hi everyone! I have behavioural videos with corresponding timestamps of trials. I would like to be able to manually select trials based on animals' behaviour. I am looking for an open-source...

05 February 2024 1,661 3 View

How long transfected cells can be cultured without G418 before they loss a plasmid?

Hello! I have a question regarding transfection stability in MCF7 and IMR32. For my experiment I need to transfect them with a plasmid with my gene of interest and Kan/Neo-gene which confers...

02 December 2023 1,626 2 View

RE: Dynamic Hardness from indentation test?

When test force is F (mN) and an indentation depth in a sample is h (µm), dynamic hardness (DH) is defined by the following formula: DH =a*F/h^2 [kgf/mm^2] For the triangular 115 indenter...

27 November 2023 5,170 1 View

Nucleus staining dissapear at the immunocytochemistry slides. Why it could be? and how to avoid it in future?

Good day. An immunocytochemical stain for CK20 of urothelium was perfored. Counterstain was Mayer`s hematoxylin (left figure). But after 1 day stain of the nucleouli dissapear (right figure). Why...

26 November 2023 1,241 2 View

How to quantify the free radicals?

Hello! I treat a polymer surface with plasma and I expect that some free radicals form on it, because it demonstrates high adsorption. How could I quantify the presence of free radicals on a...

01 November 2023 738 2 View

What's causing the smears on my second PCR product?

Hello, I have a trouble with a PCR reaction. The thing is, a PCR reaction from cDNA goes well, the first picture. But after purifying and using this DNA for the same PCR to multiple it I get the...

07 October 2023 2,398 6 View

Why is the decision time for Wiley journals so long?

The first time I submitted an article to the journal "Software: Practice and Experience". It took a month and a half to make a decision. It didn't get to peer review. The second submission took...

26 September 2023 2,420 1 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View