RAPD (Random Amplified Polymorphic DNA) is used in diversity studies. To estimate diversity among the species (especially with in the species if we need to check any variation these are best). RAPD primers are decamers arbitrarily designed available in different series. We can use any of them TA is 35C. These are decamer sequences randomly presnt in the whole genome. I answered many questions related to RAPD you can check then in my RG answers link patiently. Or check the attached and link. Any doubts feel free to ask.

RAPD (https://www.ncbi.nlm.nih.gov/probe/docs/techrapd/)

Your question was as follows

According to what RAPD primers are designed?

I don't understand how we choose RAPD primers, Is it expressed genes or no? and if so, it is expressed for what? why we investigate only one direction of it?

I answered your question in simple thinking that you understand.

I don't understand how we choose RAPD primers - to check genetic diversity

Is it expressed genes or no? Are these genes expressed for protein? - On the one hand you are saying these are primers and why you confuse again as genes!!!

why we investigate only one direction of it? - What do you mean by one direction!! You mean to say about single primer is it!!! That single primer only act as both Forward and Reverse.

I mentioned I answered many questions in RG the following are the links for your information. Read my answers you become expert, or still you did not follow, ask again.

Good luck!!

What is the mechanism of amplification in RAPD_PCR?

(https://www.researchgate.net/post/What_is_the_mechanism_of_amplification_in_RAPD_PCR)

In general, are RAPD's markers useful or not for studying genetic diversity of different species of same genus from the same location?

(https://www.researchgate.net/post/In-general-are-RAPDs-markers-useful-or-not-for-studying-genetic-diversity-of-different-species-of-same-genus-from-the-same-location)

Can anybody help in analysis of matric data of RAPD of plants?

(https://www.researchgate.net/post/Can-anybody-help-in-analysis-of-matric-data-of-RAPD-of-plantshttps://www.researchgate.net/post/Can-anybody-help-in-analysis-of-matric-data-of-RAPD-of-plants)

İs there any possibility of not having band in RAPD PCR for plant DNA ?

(https://www.researchgate.net/post/Is_there_any_possibility_of_not_having_band_in_RAPD_PCR_for_plant_DNA)

How to amplify RAPD-PCR and how to known error in PCR result?

(https://www.researchgate.net/post/How-to-amplify-RAPD-PCR-and-how-to-known-error-in-PCR-result)

RAPD analysis of binomial matrix and dendogram preparation?

(https://www.researchgate.net/post/RAPD-analysis-of-binomial-matrix-and-dendogram-preparation)

How to calculate alleles/number of loci for RAPD, ISSR and SSR markers?

(https://www.researchgate.net/post/How_to_calculate_alleles_number_of_loci_for_RAPD_ISSR_and_SSR_markers)

RAPD analysis for the in-vitro and in-vivo plant samples of the same plant species?

(https://www.researchgate.net/post/RAPD-analysis-for-the-in-vitro-and-in-vivo-plant-samples-of-the-same-plant-species)

My RAPD analysis is not working properly

(https://www.researchgate.net/post/My_RAPD_analysis_is_not_working_properly)

What quantity of DNA is required for RAPD?

(https://www.researchgate.net/post/What_quantity_of_DNA_is_required_for_RAPD)

Is it possible to get two different linked marker from one RAPD primer for the same character in two different studies?

(https://www.researchgate.net/post/Is-it-possible-to-get-two-different-linked-marker-from-one-RAPD-primer-for-the-same-character-in-two-different-studies)

Can I used RAPD PCR to distinguish between isolates of lactobacillus?

(https://www.researchgate.net/post/Can-I-used-RAPD-PCR-to-distinguish-between-isolates-of-lactobacillus)

Why do I get no amplification in my RAPD gradient PCR?

(https://www.researchgate.net/post/Why_do_I_get_no_amplification_in_my_RAPD_gradient_PCR)

How can I set up RAPD-PCR for my phages?

(https://www.researchgate.net/post/How-can-I-set-up-RAPD-PCR-for-my-phages)

Does anybody have experience with RAPD-PCR for typing bacteriophage?

(https://www.researchgate.net/post/Does_anybody_have_experience_with_RAPD-PCR_for_typing_bacteriophage)

Jetty Ramadevi !!!!!

Why you are very aggressive? It's not an appropriate way to explain something

Do you see (TA) to express the annealing temperature in the paper???

Do you know which part did I do in the paper???

I don't want your answer, delete it!!!!

You got the answer and changed the question!!! And commenting me.

I explained in a clear way, there is nothing AGGRESSIVE. If you think in that way I am sorry. Yes, I asked and surpriced you were a co-author in a paper, and thought you had an idea aboutTA. As combined work thought you would have some idea at least. If you do not want, do not read it agian. There are many people who follow RG and check/search for ideas/clues/sugestions etc. Many people able to solve their problems by contating me.

If you want you can DELETE YOUR QUESTION rather than CHANGING the question. Many people do like that. You put your new question separately. I too delete this part not the main part read carefully. Nothing to feel while learning.

Jetty Ramadevi

This is what you get?!!

I know what is annealing temperature but I don't ​know its abbreviation. This is a thing you have to accept WHILE TEACHING OR DON'T.

I change the question to tell you in a polite way to delete your answer, so I can delete the question. (This is a rule)

What exactly you don't understand!!

If you delete a part, that means your recognition that It was in an aggressive way!!

you don't have to use capital letters to deliver your point because no matter how you try, you are POINTLESS.

There are many people who follow RG, and I won't be one of them.

Is it clear now!!

I won't reply to this weird discussion AGAIN.

Dear Dr Amira Abou El-Nour . miRNAs (microRNAs) are short non-coding RNAs that regulate gene expression post-transcriptionally. They generally bind to the 3'-UTR (untranslated region) of their target mRNAs and repress protein production by destabilizing the mRNA and translational silencing. See the RG link: Article How do microRNAs regulate gene expression?

Kindly see also the following useful RG link: Article MicroRNA therapeutics: Discovering novel targets and develop...

Also check please the following very good RG link: Article MicroRNA: Biogenesis, Function and Role in Cancer

The following RG link is also very useful: Article The roles of microRNAs in epigenetic regulation