Very nice question,

we most of time observe this effect

eg. gene A shows up regulated level in test but the fold changes comes sometime 2 to 10 fold

statistics will consider it as high standard deviation and reject but actual reality is as we go on further new biological replicates we tend to observe this bi-modality if it exist,

I feel we need to observe trend or pattern consistency to make valid conclusion about gene expression

Ref: PNAS, 107.51 (2010): 22096–22101. PMC. Web. 29 Oct. 2017.

Bimodal gene expression (the distribution of gene products that has two maxima) is a cause of phenotypic diversity in genetically identical cell populations, and it is critical for population survival in a fluctuating environment

this is when expression system occurs by two modes leading to diversity of cells and possibly increased survival rates

Very nice question,

we most of time observe this effect

eg. gene A shows up regulated level in test but the fold changes comes sometime 2 to 10 fold

statistics will consider it as high standard deviation and reject but actual reality is as we go on further new biological replicates we tend to observe this bi-modality if it exist,

I feel we need to observe trend or pattern consistency to make valid conclusion about gene expression

Ref: PNAS, 107.51 (2010): 22096–22101. PMC. Web. 29 Oct. 2017.

Bimodal gene expression (the distribution of gene products that has two maxima) is a cause of phenotypic diversity in genetically identical cell populations, and it is critical for population survival in a fluctuating environment

This article might support.