Vijesh Kumar

12 Questions 6 Answers 0 Followers

Questions related from Vijesh Kumar

Can anybody suggest best method for protein protein interaction study?

Which is the best method for protein interaction study and which is of low cost. Is phage display a low cost viable method. Thanks

04 November 2019 1,617 3 View

While doing SSR can we expect other bands apart from the expected amplicons?

I am working on varietal identification of black pepper varieties using SSR markers. After running PAGE I am getting bands other than the expected amplicon size of 200 bp. I am getting bands of...

08 September 2019 5,325 3 View

How to revive old Phytophthora cultures stored in distilled water?

Can any body suggest methods to revive old Phytophthora cultures(more than 8 years old) stored in distilled water. The cultures were grown in carrot agar and the mycelial plugs are stored in vials...

06 April 2019 1,740 5 View

Can any one tell about the latest/good tools that can be used for comparative analysis of conserved motifs in a particular protein?

Which is the most latest tool or which is the good one?

19 December 2017 5,527 1 View

Can inoculation with a hemibiotrophic pathogen like Phytophthora capsici result in a bimodal pattern of gene expression ?

Upon challenge inoculation with Phytophthora capsici on Piper spp certain defense genes are induced and show a bi-modal pattern of gene expression in qPCR analysis. Is this because Phytophthora...

19 November 2017 1,190 2 View

What is meant by bimodal gene expression in real time PCR?

Please explain the bimodal pattern of expression in real time PCR. What is meant by it?

29 October 2017 277 4 View

Can we pool biological replicates while doing qRT-PCR?

Is there any publications regarding this. Pooling of biological replicates can minimize the use of SYBR green. I have heard about this but I did'nt come across any published information

28 October 2017 7,431 2 View

Do non host plant produce HR reaction?

Does HR reaction occurs in a Non-Host plant upon inoculation with a pathogen?

29 July 2017 9,437 22 View

Can we do comparitive proteomics using the normal SDS-PAGE, only through one dimension separation.....any body please help?

I am using an un inoculated leaf sample and inoculated leaf sample with Phytophthora. So I want to see the deferentially expressed proteins in the inoculated sample when compared to the un...

04 March 2016 7,042 3 View

Does anybody have any idea about the availability of free software for gene normalization in qPCR experiments?

The standard software Genorm and Normfinder I could not access freely. Kindly tell me if there is any free software and the latest version of it if available. Please its very urgent....PLS HELP

28 September 2015 4,446 4 View

How can I avoid primer dimers when designing real time PCR primers?

How can we use primer 3 plus to avoid dimer formation in our design?

01 April 2015 3,612 7 View

Conversion of primary somatic embryos into secondary somatic embryos

Hi, Can any body suggest ways to improve the conversion of primary somatic embryos derived from integument tissues in black pepper seeds into secondary embryos. I am facing difficulty in the...

01 January 1970 9,477 0 View