17 Questions 9 Answers 0 Followers
Questions related from Vijesh Kumar
Which is the best method for protein interaction study and which is of low cost. Is phage display a low cost viable method. Thanks
04 November 2019 1,671 3 View
I am working on varietal identification of black pepper varieties using SSR markers. After running PAGE I am getting bands other than the expected amplicon size of 200 bp. I am getting bands of...
08 September 2019 5,368 3 View
Can any body suggest methods to revive old Phytophthora cultures(more than 8 years old) stored in distilled water. The cultures were grown in carrot agar and the mycelial plugs are stored in vials...
06 April 2019 1,791 5 View
Genome sequence information of Pseudomonas aeroginosa from soil.
21 April 2018 8,882 3 View
Which is the most latest tool or which is the good one?
19 December 2017 5,568 1 View
Upon challenge inoculation with Phytophthora capsici on Piper spp certain defense genes are induced and show a bi-modal pattern of gene expression in qPCR analysis. Is this because Phytophthora...
19 November 2017 1,221 2 View
What is the minimum RIN value during bioanalyzer quantification of RNA, so that the RNA is eligible for down stream processes like qPCR etc?
04 November 2017 336 1 View
Please explain the bimodal pattern of expression in real time PCR. What is meant by it?
29 October 2017 328 4 View
Is there any publications regarding this. Pooling of biological replicates can minimize the use of SYBR green. I have heard about this but I did'nt come across any published information
28 October 2017 7,463 2 View
Does HR reaction occurs in a Non-Host plant upon inoculation with a pathogen?
29 July 2017 9,478 22 View
Can any one guide me about what are all the insilico analysis I can do with defense genes I have isolated or obtained through Transcriptome sequencing of a resistant variety of black pepper after...
04 May 2017 4,116 3 View
03 May 2017 7,955 21 View
I am using an un inoculated leaf sample and inoculated leaf sample with Phytophthora. So I want to see the deferentially expressed proteins in the inoculated sample when compared to the un...
04 March 2016 7,083 3 View
The standard software Genorm and Normfinder I could not access freely. Kindly tell me if there is any free software and the latest version of it if available. Please its very urgent....PLS HELP
28 September 2015 4,499 4 View
How can we use primer 3 plus to avoid dimer formation in our design?
01 April 2015 3,662 7 View
In silico work to be done for good publication.
24 April 2014 3,270 0 View
Hi, Can any body suggest ways to improve the conversion of primary somatic embryos derived from integument tissues in black pepper seeds into secondary embryos. I am facing difficulty in the...
01 January 1970 9,510 0 View
