What is Matthew's coefficient and how important is it in determination of protein structure?

More Prem Prakash's questions See All

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When I am trying to distill triethyl amine while drying it over calcium hydride. How do I design the setup to let hydrogen escape without losing NEt3?

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Why my gel electrophoresis have shadow bands? Please see the attached picture for the gel electrophoresis ?

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19 July 2024 148 6 View

What is the future scope of acoustic emission?

17 July 2024 1,510 1 View

Is the protecting group boc of the amino group stable at 37°C?

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12 July 2024 3,745 2 View

Why can't I detect the plasmon resonance angle with water?

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10 July 2024 4,719 3 View

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How can we generate topology file for water and helium system?

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24 June 2024 1,242 0 View

How the slurry is preparing for cathodes in batteries?

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Best Tools for Wrist PPG Signal Analysis: Your Recommendations?

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I need JCPDS file of LSFCO nanomaterial. Can anyone provide me?

Require for Rietveld refinement in XRD.

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Why does my protein refolded to beta sheet during thermal denaturation analysis?

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Weak DAPI staining after immunohistochemistry - how to improve?

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Please explain how the plastic input value should be considered from the true stress-strain curve for the bilinear elastoplastic material model ?

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Is it necessary to covary exogenous constructs in a structural model?

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S D Yogesha

The following paper and references there in might help you.

"Matthews coefficient probabilities: Improved estimates for unit cell contents of proteins, DNA, and protein–nucleic acid complex crystals"

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2323984/

Matthews, B.W. 1968. Solvent content of protein crystals. J. Mol. Biol. 33 491–497. [PubMed]

Ajay Wagh

Hi Prem,

This website which has some imp info about MCoe. Hope it will clear your concept about it.

http://people.mbi.ucla.edu/sawaya/tutorials/Characterize/characterize.html

Abdelhamid Eltoukhy

I am working on CD79b protein. Its R value free is 0.263, R value work is 0.181, and the resolution is 3.2 angstrom. Is the quality of the structure acceptable in this case?