I want to check the drug release percentage. 2mg initial drug, 25mL buffer solution, 2 mL solution was taken out to check absorbance by UV and replaced with 2mL fresh buffer solution, What will be the dilution factor? for the following calulation.
First Step
Determine concentration of drug released by using formula
Y =mX +/- C
Y is absorbance, m is slope, C is intercept, X is concentration (mcg/ml)
Second Step
Calculate amount of drug released
Amount of drug released (mg)= [Concentration X Dilution factor X Volume of Dissolution medium] / 1000
Final Step
% Drug release = Amt of drug released (mg) X 100 / Dose (mg)
I want to check the drug release percentage in following experiment. 2mg initial drug, 25mL buffer solution, 2 mL solution was taken out to check absorbance by UV and replaced with 2mL fresh buffer solution, What will be the dilution factor for the above calculation when calculating amount of solubilized drug in the release medium by the following formula:
Amount of drug released (mg)= [Concentration X Dilution factor X Volume of Dissolution medium] / 1000 Shafq Al-azzawi
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