Asmaa Nasr

5 Questions 5 Answers 0 Followers

Questions related from Asmaa Nasr

Why cells are stressed and how to eliminate this stress, can cells become healthy again with passage?

I am working with veroe6 for a while and every time I receive a new healthy flask one or two passages and cells become stressed. Please any one will give help why this happens and how to prevent...

20 July 2023 2,302 1 View

Can DNA ladder work well after getting expired?

I have one 100bp and 1kb dna markers in the lab nearly and their expiry was in 2015, stored in -20 since then, will they still work well?

10 November 2021 2,328 6 View

Which Tm should I use in my PCR ?

I have ordered primers from Thermo fisher scientific and in the analysis sheet it has been mentioned two tm The first one is tm 1M na+ 70 and the second is Tm 50mM na+ 48 and each has different...

30 August 2020 7,195 12 View

Does the incubation time for elution DNA affect the purity if sample ?

I am using Qiagen blood and tissue kit for extracting of DNA from ticks I elute the DNA after incubation 1minute as manufacturers instructions If I increase the elusion step to more than 1...

09 April 2019 5,541 3 View

Why I have negative results on nanodrop reading ?

When I measure the Dna concentration for 2 samples The firest sample reading was all negative value abs 260- 1.4 abs 280-0.006 260/280 eas also negative -.44 While dna amount was -1.4 The second...

05 March 2019 4,440 6 View