Asmaa Nasr

11 Questions 15 Answers 0 Followers

Questions related from Asmaa Nasr

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

11 August 2024 8,407 5 View

Can I use two IRIS in the same plasmid expression cassette ?

I am trying to express m6A protein and a fluorescence one RFP in the piggyback vector. I was thinking first of using the P2A to separate between the m6A and the RFP protein but then I was afraid...

13 March 2024 9,655 0 View

What is cause of qPCR curves to be sharp?

Hi I am getting these weird curves on qPCR Anyone knows why ? I tried to decease the primer and tablet concentration No change.

19 December 2023 5,074 2 View

Why cells are stressed and how to eliminate this stress, can cells become healthy again with passage?

I am working with veroe6 for a while and every time I receive a new healthy flask one or two passages and cells become stressed. Please any one will give help why this happens and how to prevent...

20 July 2023 2,335 1 View

Can DNA ladder work well after getting expired?

I have one 100bp and 1kb dna markers in the lab nearly and their expiry was in 2015, stored in -20 since then, will they still work well?

10 November 2021 2,340 6 View

Which Tm should I use in my PCR ?

I have ordered primers from Thermo fisher scientific and in the analysis sheet it has been mentioned two tm The first one is tm 1M na+ 70 and the second is Tm 50mM na+ 48 and each has different...

30 August 2020 7,233 12 View

What does smear mean on agarose gel when checking DNA integrity ? and how to avoid them? can storage of samples at -20 cause degradation of DNA ?

When I run a gel on the extracted DNA from my samples I always notice smear with varying degree of strength what do they mean? a picture is attached to clear out my point I store the samples...

21 September 2019 2,306 12 View

Does the incubation time for elution DNA affect the purity if sample ?

I am using Qiagen blood and tissue kit for extracting of DNA from ticks I elute the DNA after incubation 1minute as manufacturers instructions If I increase the elusion step to more than 1...

09 April 2019 5,548 3 View

Why I have negative results on nanodrop reading ?

When I measure the Dna concentration for 2 samples The firest sample reading was all negative value abs 260- 1.4 abs 280-0.006 260/280 eas also negative -.44 While dna amount was -1.4 The second...

05 March 2019 4,447 6 View

Does any one know any websites which offer periodically cases of infectious diseases?

Some websites such as CDC center for diseases control and prevention offers a section called The Morbidity and Mortality Weekly Report (MMWR). Therefore, I want any suggestions for other...

24 June 2017 7,274 31 View

How to differentiate between the Brownian motion of the Bacteria and Brownian motion of the cell debris? under the microscope in the cell culrure??

I am working with mammalian cell lines, I can see some movements under the microscope, I know about the Brownian motion that can be caused by the cell debris but the question is how to...

01 January 1970 160 1 View