most researchers use the latter
Well, it depends on your objective.
For SELEX, primer biotynylation generally used for ssDNA separation. In this case your candidate aptamers are complementary non-biotinylated strands.
However, target biotinylation is best if you want to use avidin-materials as separating matrix.
Thank you very much for your answer
I think that the careful choice and design of target, primers and library are a good start. Any suggestions?
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I would like to thank Dr Eric Dausse for having already given relevant feedback.
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On a solid surface, eg. magnetic beads or in a silica column?
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