What is better in DNA aptamer synthesis: target biotinylation or primer-library biotinylation?

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Vinayakumar Gedi

Well, it depends on your objective.

For SELEX, primer biotynylation generally used for ssDNA separation. In this case your candidate aptamers are complementary non-biotinylated strands.

However, target biotinylation is best if you want to use avidin-materials as separating matrix.

Maria Sotirios Kaponi

Thank you very much for your answer