Hi

I think MTT assay is much more better than DNA assay, although it has some drawbacks.

Thanks

We found out that MTT can be inhibited by Phenol Red like any other colormetric assay, but also by HOCL, which we use to oxidize NH2 terminal a.a. to make Ag uptake easier. HOCL inhibits s-glutathiaone transferase, which can affect the MTT reaction.

Our plan is to validate the MTT assay for sensitivity with serial dilution of positive control tumor cells in different wells. Then, we will do an ATP assay like Cell-titer Glo from Promega.

Bruce

If you are looking for DNA assays, you can try CyQuant Cell Proliferation Assay.

Thanks!