What is best ( NaOH or Tris) for adjusting the PH of ATP solution (from 3.5 to 7), for enzyme activity assay?

11 November 2017 1 916 Report

I have a purified enzyme. I need to test its activity. Enzyme requires ATP as energy currency. I have disodium salt of ATP (A2383). According to specs sheet if the solution will be prepared it would have PH 3.5. As the enzyme works between PH7.3 to 7.6, therefore I want to adjust the PH of ATP solution to neutral.

In this case what should be the best between NaOH and Tris to adjust the PH7.

Note:

Enzyme buffer already contains;

Tris (PH7.6) 40mM

KCl 25mM

MgCl2 5mM

DTT 4mM

2% Glycerol

Santhosh Gatreddi

Dear Anwar,

You can use 0.1M Tris pH 9.0 to prepare the stock solution of ATP, which may bring pH nearer to neutral.

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