Muhammad Faraz Anwar

3 Questions 11 Answers 0 Followers

Questions related from Muhammad Faraz Anwar

What is best ( NaOH or Tris) for adjusting the PH of ATP solution (from 3.5 to 7), for enzyme activity assay?

I have a purified enzyme. I need to test its activity. Enzyme requires ATP as energy currency. I have disodium salt of ATP (A2383). According to specs sheet if the solution will be prepared it...

11 November 2017 916 1 View

What should be the elution volume, for Sephacryl S200 HR chromatography column, to elute 55kDa enzyme?

I am going to use Gel filtration/Molecular sieve/Size exclusion chromatography for the purification of an enzyme(55kDa) from a mixture of proteins. The volume of mixture is 1-2 mL. Attached...

01 January 1970 9,086 12 View

How to overcome the limitation pose by Ciprofloxacin insolubility at neutral PH?

I need to perform an enzyme inhibition assay. The dye in reaction mixture is PH sensitive. The enzyme activity, because of dye limitation, can only be detected at PH 7.6 (within PH7 to 8). I am...

01 January 1970 3,992 6 View