I am working on osteoporosis mice model.I want check the level of Bone-specific alkaline phosphatase in serum of osteoporotic mice and healthy mice.
Immunoenzymatic Assay
https://www.sciencedirect.com/topics/neuroscience/alkaline-phosphatase-bone-isoenzyme
We want evaluate the effect of mangiferrin in vivo acute toxicity study and we need to prepared stock 20mg/ml.kindly suggest me best solvent.
31 December 2019 3,792 3 View
I am using real time PCR of applied bio system ( quantity studio 5). I want to check the primer efficiency. Kindly suggest me best method. Thank you
10 November 2019 1,943 7 View
Dear researcher, We are working on osteoporosis. we have induced osteoporosis in mice. Further it will targeted by different nanoformulation to manage this bone disorder. So, I want to know, 1....
10 November 2019 8,641 2 View
We want to conjugate vitamin D3 to positively charge polymeric nanoparticles. I want to known the optimum concentration of ethanol used for making the vit D3 solution.
09 October 2019 5,328 1 View
We have conjugate the siRNA to nanoparticles but we want to know that how much concentration of siRNA have loaded on nanoparticles. kindly suggest me easy and low cost technique to quantify the...
09 October 2019 6,614 4 View
Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?
03 March 2021 1,763 2 View
If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis
02 March 2021 7,670 3 View
Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...
01 March 2021 6,953 1 View
Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...
01 March 2021 9,938 3 View
Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!
28 February 2021 3,951 2 View
Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...
28 February 2021 7,310 3 View
I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.
28 February 2021 6,512 3 View
Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?
28 February 2021 3,653 4 View
Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...
25 February 2021 155 2 View
Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...
24 February 2021 1,825 3 View