I am working on osteoporosis mice model.I want check the level of Bone-specific alkaline phosphatase in serum of osteoporotic mice and healthy mice.
Immunoenzymatic Assay
https://www.sciencedirect.com/topics/neuroscience/alkaline-phosphatase-bone-isoenzyme
We want evaluate the effect of mangiferrin in vivo acute toxicity study and we need to prepared stock 20mg/ml.kindly suggest me best solvent.
31 December 2019 3,942 3 View
I am using real time PCR of applied bio system ( quantity studio 5). I want to check the primer efficiency. Kindly suggest me best method. Thank you
10 November 2019 2,145 7 View
Dear researcher, We are working on osteoporosis. we have induced osteoporosis in mice. Further it will targeted by different nanoformulation to manage this bone disorder. So, I want to know, 1....
10 November 2019 8,783 2 View
We want to conjugate vitamin D3 to positively charge polymeric nanoparticles. I want to known the optimum concentration of ethanol used for making the vit D3 solution.
09 October 2019 5,453 1 View
We have conjugate the siRNA to nanoparticles but we want to know that how much concentration of siRNA have loaded on nanoparticles. kindly suggest me easy and low cost technique to quantify the...
09 October 2019 6,797 4 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....
31 July 2024 3,756 0 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View