How to quantify the siRNA binding to Nano-particles?????

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What is the solubility of mangiferrin for Stock preparation???

We want evaluate the effect of mangiferrin in vivo acute toxicity study and we need to prepared stock 20mg/ml.kindly suggest me best solvent.

31 December 2019 3,792 3 View

How to check the primer efficiency for real time PCR ?

I am using real time PCR of applied bio system ( quantity studio 5). I want to check the primer efficiency. Kindly suggest me best method. Thank you

10 November 2019 1,943 7 View

Chemically induced osteoporosis C57/b mice model is reliable for osteoporosis study?

Dear researcher, We are working on osteoporosis. we have induced osteoporosis in mice. Further it will targeted by different nanoformulation to manage this bone disorder. So, I want to know, 1....

10 November 2019 8,641 2 View

In what percentage of ethanol used to make Vitamin D3 Solution????

We want to conjugate vitamin D3 to positively charge polymeric nanoparticles. I want to known the optimum concentration of ethanol used for making the vit D3 solution.

09 October 2019 5,328 1 View

What is best method for detection of Bone-specific alkaline Phosphatase in serum???

I am working on osteoporosis mice model.I want check the level of Bone-specific alkaline phosphatase in serum of osteoporotic mice and healthy mice.

09 October 2019 9,417 1 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Can chitosan alone can act as a reducing agent for Cu(0) nanoparticle sythesis?

[Synthesizing Cu nanoparticles without using chemical reductants]

01 March 2021 8,359 1 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How to check the uv-vis absorption of nanoparticle by surface plasmon resonance?

I try to check the uv-vis absorption of Indium nanoparticle. Now I am measuring in solution, can I measure it in the form of a film?

01 March 2021 930 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View

How can I calculate the rdf of water molecules around 'Na' ions that are closest(adsorbed) to the nanoparticle(solute)?

Hi, I want to calculate the radial distribution function for water molecules around the Na+ ions adsorbed on the surface of a spherical nanoparticle using the gromacs trajectory dynamically? For...

28 February 2021 5,650 1 View

LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

26 February 2021 4,026 5 View

Asghar Narmani

Dear researcher,

The quantitative methods for this evaluation are HNMR and TGA.

Md. Zafaryab

Please explain about TGA mediated quantification

Omar Gonzalez-Ortega

Break the interaction between rna and particles, I assume it is electrostatic. Add some salt and quantify the supernatant.

which salt kindly suggest me.