We have conjugate the siRNA to nanoparticles but we want to know that how much concentration of siRNA have loaded on nanoparticles. kindly suggest me easy and low cost technique to quantify the loaded siRNA on nanopartices.
Dear researcher,
The quantitative methods for this evaluation are HNMR and TGA.
Please explain about TGA mediated quantification
Break the interaction between rna and particles, I assume it is electrostatic. Add some salt and quantify the supernatant.
which salt kindly suggest me.
We want evaluate the effect of mangiferrin in vivo acute toxicity study and we need to prepared stock 20mg/ml.kindly suggest me best solvent.
31 December 2019 3,942 3 View
I am using real time PCR of applied bio system ( quantity studio 5). I want to check the primer efficiency. Kindly suggest me best method. Thank you
10 November 2019 2,145 7 View
Dear researcher, We are working on osteoporosis. we have induced osteoporosis in mice. Further it will targeted by different nanoformulation to manage this bone disorder. So, I want to know, 1....
10 November 2019 8,783 2 View
We want to conjugate vitamin D3 to positively charge polymeric nanoparticles. I want to known the optimum concentration of ethanol used for making the vit D3 solution.
09 October 2019 5,453 1 View
I am working on osteoporosis mice model.I want check the level of Bone-specific alkaline phosphatase in serum of osteoporotic mice and healthy mice.
09 October 2019 9,542 1 View
I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.
09 August 2024 9,894 2 View
A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.
07 August 2024 5,307 1 View
I have been using paraffin, but the deposited ZnO still detach from electrode. What is the best binder to modify graphite paste electrode with ZnO nanoparticles?
03 August 2024 4,624 3 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View
I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...
31 July 2024 2,033 1 View
Following curve related to Co0.6Mn0.4Fe2O4 Magnetic nanoparticles with extra phase a-Fe2O3. I will appreciate to help me to interpretation this spectroscopy, especially the blue line.
31 July 2024 9,140 2 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
I've attempted to use this method to synthesize copper nanoparticles. Copper nanoparticles can be synthesised using a variety of precursor materials. CuSO4, distilled water, NaOH, and EDTA are...
28 July 2024 8,027 3 View
How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?
27 July 2024 6,920 1 View
It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...
25 July 2024 8,738 0 View