How to check the primer efficiency for real time PCR ?

I am using real time PCR of applied bio system ( quantity studio 5). I want to check the primer efficiency. Kindly suggest me best method. Thank you

10 November 2019 1,943 7 View

Chemically induced osteoporosis C57/b mice model is reliable for osteoporosis study?

Dear researcher, We are working on osteoporosis. we have induced osteoporosis in mice. Further it will targeted by different nanoformulation to manage this bone disorder. So, I want to know, 1....

10 November 2019 8,641 2 View

In what percentage of ethanol used to make Vitamin D3 Solution????

We want to conjugate vitamin D3 to positively charge polymeric nanoparticles. I want to known the optimum concentration of ethanol used for making the vit D3 solution.

09 October 2019 5,328 1 View

What is best method for detection of Bone-specific alkaline Phosphatase in serum???

I am working on osteoporosis mice model.I want check the level of Bone-specific alkaline phosphatase in serum of osteoporotic mice and healthy mice.

09 October 2019 9,417 1 View

How to quantify the siRNA binding to Nano-particles?????

We have conjugate the siRNA to nanoparticles but we want to know that how much concentration of siRNA have loaded on nanoparticles. kindly suggest me easy and low cost technique to quantify the...

09 October 2019 6,614 4 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Why am i not getting solid after reacting APTES with salicylaldehyde?

The reaction of APTES with salicylaldehyde in ethanol leads to the formation of imine compound yellow solid which is mention in literature, but I am not getting yellow solid I don't know why? when...

28 February 2021 2,955 2 View