15 Questions 5 Answers 0 Followers
Questions related from Syafiqah Mhd Jamal
Hi everyone. I have some confusions on ng of DNA and microliters (ul). I've read several PCR protocols which mentioned on the ideal concentration of DNA template per reaction. I couldn't...
03 March 2019 9,862 5 View
I performed 16S rRNA PCR Analysis on a total of 6 samples. I used universal primers 27F and 1492R for this PCR analysis. 5 out of 6 samples show bands detected on 1500 bp marker, however there is...
12 December 2018 4,862 1 View
12 December 2018 6,973 1 View
I did PCR analysis for my samples (total 6 samples), I got bands for the 5 samples, however, there is one sample which the band is detected at 10,000 bp? What should I do? Is there any relations...
12 December 2018 5,912 1 View
12 December 2018 9,660 4 View
Basically I want to look for the differences between storage periods and bacterial counts. However, I don't know which tests that I can use to determine the significant differences. For example,...
12 December 2018 9,066 4 View
12 December 2018 7,457 3 View
12 December 2018 5,315 0 View
12 December 2018 2,393 1 View
12 December 2018 4,982 1 View
12 December 2018 6,288 1 View
I checked the DNA concentration after I extracted the DNA of bacteria from plates. I picked colonies on plates and resuspended in PBS before adding the Proteinase K and Buffer. I already followed...
09 September 2018 2,341 9 View
Basically, I want to perform a 1:10 dilution of dry plant extracts for food contamination test. Do we have to filter to make a stock solution before proceed to the serial dilution?
07 July 2018 5,507 6 View
Specifically, I want to detect Salmonella enterotoxin gene and E. coli heat stable enterotoxin gene. However, I am confuse which PCR that I can use to detect those toxic genes. What primers should...
05 May 2018 6,462 1 View
Gamma irradiation is a method to reduce the microbial contaminants in medicinal plant extracts besides to have a safe product for the consumers. However, is there any possibilities that the...
03 March 2018 6,640 3 View
