Syafiqah Mhd Jamal

15 Questions 5 Answers 0 Followers

Questions related from Syafiqah Mhd Jamal

Nanogram (ng) of DNA is equal to how many microliters (ul)?

Hi everyone. I have some confusions on ng of DNA and microliters (ul). I've read several PCR protocols which mentioned on the ideal concentration of DNA template per reaction. I couldn't...

03 March 2019 9,819 5 View

What is exactly happened when I got DNA band on gel electrophoresis and it is detected at 10,000 bp marker?

I performed 16S rRNA PCR Analysis on a total of 6 samples. I used universal primers 27F and 1492R for this PCR analysis. 5 out of 6 samples show bands detected on 1500 bp marker, however there is...

12 December 2018 4,833 1 View

What is exactly happened when I got DNA band on gel electrophoresis and it is detected at 10,000 bp marker?

I performed 16S rRNA PCR Analysis on a total of 6 samples. I used universal primers 27F and 1492R for this PCR analysis. 5 out of 6 samples show bands detected on 1500 bp marker, however there is...

12 December 2018 6,944 1 View

What is actually happen when I got band detected on gel electrophoresis at 10,000 bp?

I did PCR analysis for my samples (total 6 samples), I got bands for the 5 samples, however, there is one sample which the band is detected at 10,000 bp? What should I do? Is there any relations...

12 December 2018 5,886 1 View

What is actually happen when I got band detected on gel electrophoresis at 10,000 bp?

I did PCR analysis for my samples (total 6 samples), I got bands for the 5 samples, however, there is one sample which the band is detected at 10,000 bp? What should I do? Is there any relations...

12 December 2018 9,633 4 View

Hi all. I have a question on statistical analysis. How we want to calculate the differences between storage periods with bacterial counts?

Basically I want to look for the differences between storage periods and bacterial counts. However, I don't know which tests that I can use to determine the significant differences. For example,...

12 December 2018 9,038 4 View

What is exactly happened when I got DNA band on gel electrophoresis and it is detected at 10,000 bp marker?

I performed 16S rRNA PCR Analysis on a total of 6 samples. I used universal primers 27F and 1492R for this PCR analysis. 5 out of 6 samples show bands detected on 1500 bp marker, however there is...

12 December 2018 7,424 3 View

What is exactly happened when I got DNA band on gel electrophoresis and it is detected at 10,000 bp marker?

I performed 16S rRNA PCR Analysis on a total of 6 samples. I used universal primers 27F and 1492R for this PCR analysis. 5 out of 6 samples show bands detected on 1500 bp marker, however there is...

12 December 2018 5,289 0 View

What is exactly happened when I got DNA band on gel electrophoresis and it is detected at 10,000 bp marker?

I performed 16S rRNA PCR Analysis on a total of 6 samples. I used universal primers 27F and 1492R for this PCR analysis. 5 out of 6 samples show bands detected on 1500 bp marker, however there is...

12 December 2018 2,363 1 View

What is exactly happened when I got DNA band on gel electrophoresis and it is detected at 10,000 bp marker?

I performed 16S rRNA PCR Analysis on a total of 6 samples. I used universal primers 27F and 1492R for this PCR analysis. 5 out of 6 samples show bands detected on 1500 bp marker, however there is...

12 December 2018 4,956 1 View

What is actually happen when I got band detected on gel electrophoresis at 10,000 bp?

I did PCR analysis for my samples (total 6 samples), I got bands for the 5 samples, however, there is one sample which the band is detected at 10,000 bp? What should I do? Is there any relations...

12 December 2018 6,263 1 View

What should I do if I don't get any band when checking the DNA concentration by viewing gel?

I checked the DNA concentration after I extracted the DNA of bacteria from plates. I picked colonies on plates and resuspended in PBS before adding the Proteinase K and Buffer. I already followed...

09 September 2018 2,325 9 View

What method I can use to dilute the dry plant extracts for food contamination test?

Basically, I want to perform a 1:10 dilution of dry plant extracts for food contamination test. Do we have to filter to make a stock solution before proceed to the serial dilution?

07 July 2018 5,488 6 View

What PCR I can use to detect toxic genes from Salmonella sp and E. coli?

Specifically, I want to detect Salmonella enterotoxin gene and E. coli heat stable enterotoxin gene. However, I am confuse which PCR that I can use to detect those toxic genes. What primers should...

05 May 2018 6,444 1 View

Is there any possibilities for bacteria contaminants in medicinal plants produce toxins after the exposure to gamma radiation?

Gamma irradiation is a method to reduce the microbial contaminants in medicinal plant extracts besides to have a safe product for the consumers. However, is there any possibilities that the...

03 March 2018 6,625 3 View