How do plants differentiate between virulent and avirulent microorganisms?

For example: All bacteria prodcur Flagellin that can be detected by the plant to elicit a immune response. How does the plant 'know' which bacteria beneficial or virulent and when to orchestrate...

21 January 2020 5,139 3 View

How to remove the cuticular wax layer off of plant foilage?

I would like to remove the wax layer off of Lactuca sativa (Lettuce) and perform a ROS assay on the leaf discs. Does anyone know how to remove the wax without disturbing the integrity of the...

31 December 2019 9,163 3 View

What is an appropriate solvent for chitin?

I want to inject the chitin solution into the foliage of lettuce and measure a possible difference is reactive oxygen species production. Conventional solvents are often toxic or corrosive which...

10 November 2019 743 3 View

What is an appropriate solvent for chitin?

I want to inject the chitin solution into the foliage of lettuce and measure a possible difference is reactive oxygen species production. Conventional solvents are often toxic or corrosive which...

06 November 2019 8,552 4 View

What is a suitable way to extract phytophthora mycelium from liquid media?

The mycelium nearly completly covers the whole 'pea-media'. The strain has been growing at 28oC, not shaking.

28 October 2019 6,549 4 View

How can I explain a decline in OD600?

03 June 2019 6,830 2 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Can water be extracted from Olivine?

I have always been interested in geology and wondered about how unique structures of materials behave in different circumstances. I stumbled upon Olivine, the magnesium iron silicate, and heard...

28 February 2021 7,330 4 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

How to arrange coordinates and extract climate dataset out of MPI-ESM-MR netcdf files?

Have a nice day everyone, I'm stuck in extracting datasets out of MPI-ESM-MR netcdf files because the latitude ranges from -3*10^6 to 3*10^6 and longitude ranges -5,9*10^6 to 5,9*10^6. Plus, the...

28 February 2021 912 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View