Seyed Mahyar Mirmajlessi

3 Questions 11 Answers 0 Followers

Questions related from Seyed Mahyar Mirmajlessi

What is the best way of making solutions from Ethanol, Ethyl Acetate and Hexane extractions?

I have a different small amount of Ethanol, Ethyl Acetate and Hexane extractions originated from plant materials as bioproducts and am going to make solutions in order to spray on the plants. Can...

10 October 2018 3,240 3 View

Does the volume of sample in the tube is really important for phases separation (aqueous phase) when using TRI Reagent protocol for RNA extraction?

We have crushed plant samples in micro-tube (2 ml) which previously filled in until less than half. After adding TRI reagent, chloroform, and centrifuging, we do not get aqueous phase!, only...

10 October 2018 1,545 2 View

What could be the reason when you have problem with standard curve in qPCR test?

qPCR (TaqMan) assay was done to set up a standard curve. Only the first three diluted points (10ng/ul,1ng/ul, and 0.1ng/ul) of our standard curve amplifies - every 1:10 dilution after that (0.01...

03 March 2018 2,318 10 View