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Questions related from Tom de Kanter
We are trying to create a method to separate high and low lipid-producing yeast cells after EMS treatment (mutagenesis). We have seen cells that accumulate up to 70% of their dry-weight in lipid...
16 March 2021 9,087 1 View
For example: All bacteria prodcur Flagellin that can be detected by the plant to elicit a immune response. How does the plant 'know' which bacteria beneficial or virulent and when to orchestrate...
22 January 2020 5,221 3 View
I would like to remove the wax layer off of Lactuca sativa (Lettuce) and perform a ROS assay on the leaf discs. Does anyone know how to remove the wax without disturbing the integrity of the...
01 January 2020 9,239 3 View
I want to inject the chitin solution into the foliage of lettuce and measure a possible difference is reactive oxygen species production. Conventional solvents are often toxic or corrosive which...
11 November 2019 815 3 View
07 November 2019 8,615 4 View
The mycelium nearly completly covers the whole 'pea-media'. The strain has been growing at 28oC, not shaking.
29 October 2019 6,603 4 View
10 October 2019 3,158 5 View
I inoculated a WT Saccharomyces serevisiae strain in liquid minimal media at 30 C, 250 rpm. I meassured the OD600 every 20 min and after a ´normal´ increase in OD, the OD suddenly drops. Does...
04 June 2019 6,936 2 View
We performed transformation on Saccharomyces cerevisiae, where we introduced a gene mutant library (roughly 14 mil. mutants). Because of the high transformation efficiency, many colonies appeared...
01 May 2019 5,091 3 View
