Linear regression assumption violation?

I am trying to perform linear regression, but my response variable seems not to follow a normal distribution (rejected H0 in ks test) with positive skewness (the distribution is attached - Fig1)....

07 August 2019 5,160 21 View

How to calculate gene expression fold change among multiple conditions?

Hi, This seems a bit unusual to me, since I could not find any related paper. This is my situation: We have CT values (3 replicates each) for following conditions for gene A: - Wild type (WT)...

01 February 2019 5,299 6 View

How to employ hypergeometric test correctly?

Hi, Lets give a vivid example to explain my question: suppose we have a list of universal genes including both oncogenes (OG) and tumor suppressor (TS), and the purpose is to test the enrichment...

06 July 2017 5,853 3 View

How to interpret RMA log2 intensity data?

Hi! I am confused with some terms, so, I appreciate any help in advance. Array data sets are usually reported as intensity levels of probes (expression values). Some researchers report their data...

03 April 2017 1,788 0 View

Can two/more isozymes catalyze the same reaction at the same time?

Is it possible for a cell to have two or more isoenzymes catalyzing a biochemical reaction at the same time? For instance, we know that two alcohol dehydrogenase (ADH) isoenzymes simultaneously...

11 December 2016 6,394 4 View

Repeated N-fold cross validation?

Hi! I am a bit confused with the term of "repeated" in repeated CV. In a typical cross validation problem, let's say 5-fold, the overall process will be repeated 5 times: at each time one subset...

10 November 2016 7,871 4 View

How to reconcile multiple gene ID mapping when dealing with probe set id conversion?

I want to get Entrez IDs for Affymetrix probe sets (hgu133a) to map them on genome-scale metabolic models (GSMMs). Generally, GSMMs use Entrez gene IDs; therefore, to integrate gene expression...

06 July 2016 6,763 3 View

Where can I find a complete list of oncogenes and TS for NCI-60 cell lines?

01 February 2016 3,348 2 View

Which parameters affect the choice of a tumor cell line?

There are various cell lines in literature from different cell collections (ATCC, DSMZ, etc.) or personal donations extracted from patients or just a gift from a generous researcher. However, not...

08 September 2015 5,523 7 View

How can we concentrate small volumes of a protein solution?

Is there any special centrifuge filter or another instrument for the purpose of tiny volumes?

04 May 2013 3,322 16 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View