What is a suitable CSI protocol for detecting glutamate in human glioma?

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My question is about errors in Ansys mechanical APDL? Data file file.db does not exist for RESUME?

I did a welding simulation using ANSYS MECHANICAL APDL. The solution was done, but I encountered this error in the output file (Data file file.db does not exist for RESUME). On the other hand, I...

11 July 2024 4,480 0 View

What Remote Sensing Software Is User-Friendly for Urban Ecology Research?

Hello everyone, Our lab is currently looking to purchase user-friendly remote sensing software, particularly for individuals without a background in remote sensing or coding. Ideally, we are...

05 June 2024 2,940 6 View

By which acid can we replace sulfamic acid to eliminate nitrite in a soil extract to determine the nitrate content by the DEVARDA method?

by which acid can we replace sulfamic acid to eliminate nitrite in a soil extract to determine the nitrate content by the DEVARDA method

26 May 2024 5,822 1 View

Could you provide me with the ISO protocol for measuring nitrites and nitrates in soil?

Could you provide me with the ISO protocol for measuring nitrites and nitrates in soil?"

25 May 2024 252 0 View

Nitrate dosage KCl?

Can I apply the nitrate dosage method in water to the soil extract with KCl?

25 May 2024 8,169 4 View

How we can add PIN Diode in CST ??

Hello All i want to design a reconfigurable antenna for which i have to use PIN diode as a switch. anyone help me how to add PIN diode in CST i mean in schematic ?? Thank you

04 March 2024 8,098 1 View

I have a new article and I want to publish it in your journal , how can I do this?

I have a new article and I want to publish it in your journal , how can I do this? I have defended my dissertation recently and taken a short article from it

14 February 2024 673 3 View

CO and Cross polarization using HFSS or cst?

Greetings, Please how can i plot Cross or CO polarization on both hfss and cst Thank you

15 October 2023 4,393 2 View

Immunofluorescent marker for SH-SY5Y cells?

Hi everyone. we are doing IF for checking the translocation of a protein between cytoplasm and nucleus in SY5 cells , and I need a marker better than NeuN to visualize the whole cell and make it...

07 August 2023 9,942 4 View

Protocol for an acid protease test?

Hi everyone What is the protocol for an acid protease test, excluding the use of hemoglobin? I am seeking cost-effective testing protocols. #acid protease #protocol

29 July 2023 4,145 3 View

What are the emerging trends and opportunities for STEM education and careers in the online space?

10 August 2024 507 0 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

How can I get my Granzyme B flow cytometry stain to be consistent?

I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...

01 August 2024 7,677 2 View

Best mask design for metal deposition aiming build a Perovskite solar cell device?

What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?

31 July 2024 4,835 3 View

How to culture and maintain Lx-2 cells?

Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...

31 July 2024 3,893 0 View

How to treat monolayer culture cell line with essential oil?

Hi all, may I ask you for advice about treating monolayer culture cell line with essential oil. Due to the hydrophobic character of the oil, I am concerning how can it equally affect to all the...

31 July 2024 5,301 2 View