Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

Why can`t I isolate RNA from Extracellular vesicles?

Hello, I have been trying to isolate RNA from EVs (from MSCs) for a while. I isolate the EVs using SEC. Then I use the "Total Exosome RNA and Protein Isolation Kit" from Invitrogen. When I...

21 October 2020 8,559 4 View

How to directly use a directory of .h5 files as the training set in Python without appending them to a list?

I am trying to append .h5 files saved in a directory for my training set using Python, but I face memory error as the Data set is very large. The following code is what I used for...

23 April 2020 9,649 2 View

To record trajectory tracking performance of a controller, is it resonable to calculate the mean_square_error or the normalized-measn-square-error?

I some papers, the mean square error is considered. In some other, the mse is normalized by dividing the error by the (total sampling instants x the total length of the reference...

25 February 2020 784 4 View

What is the most reliable guide for identification of alternaria species based on morphological and cultural criteria?

what is the most reliable guide for identification of alternaria species based on morphological and cultural criteria?

02 December 2019 174 1 View

How the nonholonomy of mobile robots allow control of the system with less control inputs?

In "Simultaneous Stabilization and Tracking of Nonholonomic Mobile Robots: A Lyapunov-Based Approach", the authors stated that the nonholonomy of mobile robots allows control of the system with...

27 May 2019 7,721 4 View

How to interpret DMA?

18 May 2019 631 6 View

What are the most reliable tools or softwares for designing of the CRISPR gRNAs?

Dear all, Which tool is more reliable? CHOP CHOP or CRISPRdirect? I need some information about other tools and softwares. Thanks a lot

08 May 2019 10,004 2 View

If you could recommend one significant change to make Higher education more affordable without compromising its worth, what would it be?

Higher Education cost is increasing and students encounter some challenges to afford it.

21 March 2019 7,173 6 View

How to match reflection in both visible and infrared?

I have a thin film of a fluorescent material and want to make a reference sample with similar reflection in both visible and infrared to do the comparison. How can I make the reference sample?

26 February 2019 6,857 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View