10 October 2014 1 6K Report

I want to make a coupling of siRNA to a peptide. So, first I want to blend siRNA sense and antisense together to prepare the siRNA. I want to know the concentration of each strand.

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Does anyone know the best method to storage the whole mouse brain?

I will be removing whole mouse brains to be later used. So, I need to know the best methods to store the brain.

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Could anyone suggest a suitable detergent to use to solubilize SKCa2 in mouse brain tissue?

I am searching for a suitable detergent to solubilze SKCa2 channel in mouse brain tissue.

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Could anyone suggest an antibody for western blot analysis to SKCa2 in mouse brain tissue?

I am searching for an antibody suitable for SKCa2 channel.

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Does anyone have an idea how to resolve the problem of cation exchange in chromatography salts?

I did cation exchange chromatography with buffer A (sodium acetate) and buffer B (sodium acetate+NaCl). Now I have a problem, I can not do MS because I have a high concentration of salt in my...

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