I want to make a coupling of siRNA to a peptide. So, first I want to blend siRNA sense and antisense together to prepare the siRNA. I want to know the concentration of each strand.
The sense and antisense comes together as a duplex. Using separate SS and AS is currently outdated (as they are not stable enough).
Do you like to couple your peptide with the AS strand and then mix it with SS? or the duplex with protein?
Thank you for your answer. Yes, I will use the duplex with protein.
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