What is the reason for change in mRNA level of specific gene but not change in protein level?

With treatment of cell lines ( cardiofibroblast cells), changes in mRNA level of the gene of interest has been observed overtly, but there is no change in protein level. I am wondering how this...

07 August 2017 1,964 7 View

How can point mutation in a protein affect amount of other protein in cells?

It has shown that about gene knock out in mice'brain, the amount of other protein can be affected (Maybe in mRNA level or protein stability). I want to know whether point mutation can lead to the...

04 May 2017 3,735 4 View

Have anybody experience in taking special GRE in biochemistry?

I'm looking for a set of books that I can read in preparation for the GRE Subject Test In Biochemistry, Cell And Molecular Biology

08 September 2015 8,014 1 View

Can anybody introduce books in field of oncology?

I want to know oncology books that is comprehensive. I want to know from beginner to advanced. I need materials that is useful for molecular medicine major.

06 July 2015 8,964 3 View

Does rigidity structure of enzyme always lead to decrease enzyme activity?

Article show that rigidity affects enzyme activity and high rigidity causes low activity. Is there any exceptions? How does increasing rigidity cause high activity?

01 February 2015 9,685 11 View

What is the effect of immidazole on activity and stability of enzyme?

When enzymes were eluted with high concentration immidazole, some of them lost their activity in 2 days. I want to know how immidazole affect enzyme or other protein. I can't find article about that.

31 December 2014 8,559 7 View

How does the removal of a small domain from an enzyme affect the optimum PH of activity?

my enzyme is cellulase. It has two domains( small domain and catalytic domain). small domain has a few hydrophobic interaction with catalytic domain. I determined PH optimum for truncated and...

11 December 2014 8,513 4 View

How can I assay the aggregation of my enzyme?

I removed small domains from my enzyme. I want to know which one (truncated or wild-type) is more likely to aggregate at different times at 65 centigrade. Can you give me a strategy to go about...

11 December 2014 5,926 9 View

Why do truncated and wild-type enzyme have different colors in bradford assay with the same sharpness in SDS-PAGE?

I remove 85 aa from my enzyme(cellulase). I purified enzymes(truncated and wild-type). After dialysis, I run 20 microliter of them for SDS-PAGE. Both of them have the same sharpness on gel, but...

10 November 2014 7,843 4 View

Why did my protein elute with wash buffer (even 10 mM imidazole)?

I can't purify my protein with the ni-agarose column. The PH wash and elution buffer is ok but all of my protein eluted in the wash buffer. What is my problem?

09 October 2014 981 17 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

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02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View