I am designing a PCR to detect the presence of Proteus vulgaris. Unfortunately, all the literature I have come across use either the 16S rRNA gene or the 16S-23S ITS. Does anyone know of a virulent or structural gene specific only to Proteus vulgaris?
Zh Mikrobiol Epidemiol Immunobiol. 2005 May-Jun;(3):33-9.
[Species-specific detection of Proteus vulgaris and Proteus mirabilis by the polymerase chain reaction].
[Article in Russian]
Limanskiĭ A, Minukhin V, Limanskaia O, Pavlenko N, Mishina M, Tsygenenko A.
Abstract
Sets of primers for the species-specific detection of P. mirabilis and P. vulgaris by the polymerase chain reaction (PCR) were developed. As targets for these primers beta-lactamase and 16S rRNA gene fragments were chosen on the basis of the multiple leveling of the sequences of the DNA of all known P. mirabilis and P. vulgaris isolates.
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