Am not sure but I think companies can modified 3' end of your primer. I suggest that you ask primer design companies. If you need a surgeon in a company PM back
Many of the companies I know can attach 3' terminators to inhibit pcr extension at the 5' end, but I haven't come across any modifications that can be cleaved or switched off to re-activate polymerase.
Hi Jones, I will have a look with out company to see if is possible, and i will come back to you
HI Jones
There are some photocleavable groups available that are attached sometimes to DNA molecules including primers....check on internet if such chemicals are available for 3' modifications also.
bye
To prevent the amplification we can modify the primer at 3' end. So that the 3' modification the primer prevents the extension. This can be achieved by replacing of the 3'-OH group of primer with phosphate (P04) group (phosphorylation). Hence, the 3'-OH group is necessary for primer extension. Hence, the modified primer doesn't contain the free 3'-OH group and it prevents the primer extension. When we want amplification, just treat with phosphatase (Antarctic phosphatase) which removes the phosphate group and the primer is ready for extension.
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