What is a simple method for gene deletion and complementation in Bacillus subtilis?

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Strong and extreme winds in the Arctic?

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25 January 2021 8,984 4 View

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Dear Colleagues, I need your help. In book (can be found in Internet, free) Todhunter, I., & Pearson, K. (1893). A History of the Theory of Elasticity and of the Strength of Materials (Vol....

26 December 2020 433 1 View

Combine several datasets?

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31 October 2020 4,875 2 View

Does the quantum vacuum have analogies in mathematics?

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13 September 2020 8,593 13 View

Can AI be emotionally intelligent (whatever AI, emotional, or intelligent may mean to you)?

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11 September 2020 5,158 5 View

Where was Fischerella sp. PCC 9339/Fischerella musciola (UTEX1829) isolated?

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Will DNA degradate if left over weekend during tissue lysis in extraction methods ?

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09 July 2020 4,084 4 View

Are these claims about compactification true?

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Possible optimization algorithms for fitting a nonlinear model with no analytic solution (contains integrals that can only be solved numerically)?

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14 April 2020 2,117 12 View

How can I design a PBG substrate microstrip antenna in Matlab?

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10 April 2020 7,257 4 View

How to normalise data on OPM package from R Studio ?

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02 March 2021 3,584 1 View

How to repeat an operation on an atom selection for multiple times in PyMol?

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What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

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Is there a powerful system for the security of the systems distributed on IoT systems?

02 March 2021 3,858 10 View

How Relativistic effects is one reason for lanthanide contraction?

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01 March 2021 2,272 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

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01 March 2021 3,607 3 View

Genome-wide gene identification protocol ?

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28 February 2021 8,095 1 View

For modelling epoxy-based matrices (in composites), which model in ABAQUS is the most suitable, Concrete damaged plasticity or Drucker-Prager model?

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Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

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27 February 2021 356 6 View

Can anyone share his experience regards Smoothing in origin?

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26 February 2021 8,183 3 View

Magda Luciana Atilano

You can use a vector designated pMAD that was constructed for quickly generating gene inactivation mutants in gram-positive bacteria including B. subtilis. I use this frequently for S. aureus bacteria but a couple of friemds tested for bacilus with great results. You can also use the same vector to make complementation. Check this paper from Arnaud et al 2004: doi: 10.1128/AEM.70.11.6887-6891.2004. Good luck

Mark K Chee

I have heard from people who use B. subtilis as a model system that this bacterium is naturally competent and very easy to work with. I can suggest a few contacts if you need them.