Amit Kulkarni

3 Questions 13 Answers 0 Followers

Questions related from Amit Kulkarni

What are minimum nucleotides of U6 promoter required to drive shRNA expression ?

I want to construct a vector to drive shRNA expression by U6 promoter. However, due to limited genetic space I would like to use minimum possible nucleotides of U6 promoter. Do anybody know...

10 February 2017 5,071 2 View

Can anyone recommend using a lentivirus based vector for cloning 5 kb orf and it's expression?

I am looking for lentivirus based system to clone 5 kb orf gene (membrane protein). From my reading, it appears to me that a lentivirus system with 4kb insert and above is unstable and...

16 October 2014 8,486 2 View

What is best transfection reagent for co-transfection or sucessive transfection of siRNA and DNA in Hela cells ?

I was wondering if someone has optimised a protocol for co-transfection of DNA and siRNA in Hela cells ? I am attempting a rescue experiment were siRNA treated Hela cells are transfected with the...

11 February 2014 4,216 8 View