How do i search for a dissertation?

I was referred her because i wanted to ask the author to view the dissertation The Housing that Model Cities Built: Context, Community and Capital in New York City, 1966-76, Schindler Killian...

16 December 2020 5,849 6 View

How to prepare a 0.2 M phosphate buffer?

I need to prepare a 0.2 M phosphate buffer. In my research, I've seen people prepare it with both monobasic and dibasic sodium phosphate... is this necessary? Can I just use one or the other?

13 September 2020 4,656 2 View

Non-parametric equivalent of three-way ANOVA for small sample size?

I inherited a dataset with a very small sample size (n=3 per group) and three factors (primary insult x secondary insult x treatment). I intended to use a three-way ANOVA, but I was warned that...

31 March 2020 5,913 3 View

How do I obtain a doi for my publication?

A researcher here at my institution is asking about getting a doi for his publication to increase its findability and exposure. Does one have to go through the avenue of a Registration Agency? If...

04 February 2020 8,760 6 View

Does genomic DNA bands smear on gel are degraded DNA?

I have to send my sample for metagenomics sequencing but my genomic DNA shows up a clear band with thin and long smear. It looks like my sample has degraded but I'm not sure. I read somewhere that...

15 December 2019 206 3 View

How to summarize reliability values ?

Hello, I am coding some metrics from different articles to run a meta-analysis and I had a simple question. Let's say one of my variable of interest is Brand loyalty. In some articles, brand...

19 August 2019 171 2 View

Ways to dry phenolic extracts in 70% acetone (2mL samples in 96 deep well plates)?

We are extracting phenolics using 70% acetone. The samples are set up in 96 deep well plates. The CentriVap (rotary vacuum) and lyophilizer that we have are made with materials that could be...

02 September 2018 3,880 3 View

Was the heavy cavalry of Alexander the Great the first cavalry to charge home in combat against unbroken enemy cavalry?

18 August 2018 7,200 6 View

Methods for sterilizing (killing biota on) granular activated carbon (GAC) ?

I am only interested in methods that don't change the GAC's surface chemistry or absorption capacity. The ionising and non-ionising irradiation methods we have looked into seem likely to affect...

03 April 2018 5,313 3 View

Probability of viral plaques to skip a dilution at low levels?

Hello all, I'm looking to calculate the probability to have viral plaques appear in lower dilutions but not in the previous dilution. We perform serial 10 fold dilutions (0.2 ml into 1.8ml...

20 February 2018 2,350 2 View

Anyone that can help with Maxquant TMT16plex which is missing?

Hi I am very new to proteomics analysis. I have got raw files from core facility running thermofisher TMT 16plex 4 hours. I cannot find on maxquant the option TMT16Plex there is only up to 11...

25 February 2021 1,675 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

What is the minimum number of exosomes/ml to have a good yield of total RNA for Illumina sequencing?

I am using IZON qEV2 columns to isolate exosomes from 100 ml of cancer cell conditioned medium. in NTA analysis I usually see concentration of 2x10^9 particles/ml. But when I proceed with this...

20 February 2021 6,879 3 View

Unable to detect a truncated protein variant, but I can observe a truncated cDNA from Sanger?

Hi, so basically... I am trying to mimic a clinically observed condition wherein a frame-shift mutation happens only at one allele of the patients, giving rise to a truncated variant which evades...

17 February 2021 1,130 6 View

Is it possible to remove the cells from a tissue and keep only the extracellular matrix for proteomics?

Hello! I would like to know if there is a way to flush/remove all the cells out of a tissue and keep only the extracellular matrix, in order to analyse its composition using e.g. proteomics....

14 February 2021 1,602 4 View

Apart from MOFA what other software is available for analysis of a multi-omic study?

Study bringing together genomics, proteomics etc together

02 February 2021 2,856 2 View

How can I reduce run-to-run variation in TMT MS experiments?

I planned an experimental setup using 10-plex TMT with 9 conditions and 1 bridge channel to compare 5 replicates. But since I experienced some run-to-run variation, I was wondering if a better...

31 January 2021 3,405 3 View

Where can I purchase NanoLC emitter fused silica picoTip?

Hi, I'm working with NanoLC/MS for both Metabolomics and Proteomics. We usually make our nano columns using emitters from NewObjective. Unfortunately New Objective, due to the pandemic, is now...

10 January 2021 1,176 4 View

An optimal protocol of EVs isolation for proteomics?

Hi everyone, I am starting a project in analyzing EVs proteomics in cancers. However, the most published articles I have found stated only brief isolation protocols that could not fully be...

17 December 2020 7,600 2 View

Has anyone used Isolate II RNA mini kit for isolation miRNA from exosomes?

I will be isolating exosomes from plasma with Total Exosome Isolation Kit (Invitrogen). Will Isolate II RNA mini kit (Bioline) be appropriate for miRNA isolation from obtained exosomes? The...

26 November 2020 9,503 5 View