20 September 2023 2 9K Report

I'm currently working with human primary T cells, isolated from whole blood that I get from my university's blood bank, and I'm trying to determine if my experimental treatment can enhance cytotoxicity. I'd like to run some type of in vitro killing assay but am struggling to find a good target cell for the cells that I work with.

In general, I use cells that are not CMV-positive. I've seen lots of literature using T cells from OVA mice and having the target cells pulsed with OVA peptide, but something like this isn't feasible with the cells that I have access to. Does anyone know if it's possible to run a killing assay of this type with run-of-the-mill human T cells? Is there a good target cell line that any human T cell with kill?

Thanks in advance!

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